Application | Comment | Organism |
---|---|---|
industry | the huge production of engineered GLA produced in Escherichia coli under optimized conditions makes this enzyme an economic tool to be used in bioconversion processes at the industrial level, e.g., in 7-ACA production | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
into the vector pET24 for expression in Escherichia coli BL21DE3 pLysS cells | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
alpha-subunit, determined by SDS-PAGE | Pseudomonas sp. |
58000 | - |
beta-subunit, determined by SDS-PAGE | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | Pseudomonas sp. | - |
(7R)-7-aminocephalosporanate + glutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by HiTrap chelating affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
LB Miller broth medium | Pseudomonas sp. |
3.7 | - |
purification step crude extract | Pseudomonas sp. |
11.4 | - |
optimized conditions, SB3 medium, 12.5 g NaCl/l, 0.6 mM IPTG | Pseudomonas sp. |
125 | - |
purification step HiTrap chelating chromatography | Pseudomonas sp. |
133 | - |
purification step PD10 desalting | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | - |
Pseudomonas sp. | (7R)-7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | activity assay | Pseudomonas sp. | 7-aminocephalosporanic acid + glutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
glutaryl-7-aminocephalosporanic acid acylase | - |
Pseudomonas sp. |