Application | Comment | Organism |
---|---|---|
synthesis | bioconversion of cephalosporin C into 7-aminocephalosporanate | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
A215E | Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 7.6fold lower than wild-type value | Pseudomonas sp. |
A215F | Vmax/Km for cephalosporin C is 2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 3.9fold lower than wild-type value | Pseudomonas sp. |
A215L | Vmax/Km for cephalosporin C is 1.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 4.2fold lower than wild-type value | Pseudomonas sp. |
A215V | Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.5fold lower than wild-type value | Pseudomonas sp. |
A215Y | Vmax/Km for cephalosporin C is 4.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.6fold lower than wild-type value | Pseudomonas sp. |
A215Y/H296S | Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 25.1fold lower than wild-type value | Pseudomonas sp. |
A215Y/H296S/H309S | Vmax/Km for cephalosporin C is 3.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 13.7fold lower than wild-type value | Pseudomonas sp. |
A215Y/H309S | Vmax/Km for cephalosporin C is 3.8fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 2.9fold higher than wild-type value | Pseudomonas sp. |
D416Y | Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.2fold lower than wild-type value | Pseudomonas sp. |
D416Y/H417Y | Vmax/Km for cephalosporin C is 5.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 151fold lower than wild-type value | Pseudomonas sp. |
E89A/A215Y | Vmax/Km for cephalosporin C is 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 25.2fold lower than wild-type value | Pseudomonas sp. |
F270M | Vmax/Km for cephalosporin C is 1.8fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.7fold lower than wild-type value | Pseudomonas sp. |
H296F | Vmax/Km for cephalosporin C is 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 50.3fold lower than wild-type value | Pseudomonas sp. |
H296N | Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is fold lower than wild-type value | Pseudomonas sp. |
H296S | Vmax/Km for cephalosporin C is 1.7fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 10.1fold lower than wild-type value | Pseudomonas sp. |
H296S/H309S | Vmax/Km for cephalosporin C is 4fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 21.6fold lower than wild-type value | Pseudomonas sp. |
H296S/H417M | Vmax/Km for cephalosporin C is 2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 151fold lower than wild-type value | Pseudomonas sp. |
H296T | Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.2fold lower than wild-type value | Pseudomonas sp. |
H309S | Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 8.9fold lower than wild-type value | Pseudomonas sp. |
H309Y | Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 16.8fold lower than wild-type value | Pseudomonas sp. |
H417Y | Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 4.4fold lower than wild-type value | Pseudomonas sp. |
I44V/E49stop/D416Y/H417Y | Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is more than 1510fold lower than wild-type value | Pseudomonas sp. |
S22P/T394P/D416Y/H417Y | Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is more than 1510fold lower than wild-type value | Pseudomonas sp. |
Y215Y/F270S | Vmax/Km for cephalosporin C is 2.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.2fold lower than wild-type value | Pseudomonas sp. |
Y271F | Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.4fold lower than wild-type value | Pseudomonas sp. |
Y27V | Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 2.1fold lower than wild-type value | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296S | Pseudomonas sp. | |
0.9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215F | Pseudomonas sp. | |
0.9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215Y/H309S | Pseudomonas sp. | |
1 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215E | Pseudomonas sp. | |
1 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H309Y | Pseudomonas sp. | |
1.1 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme E89A/A215Y | Pseudomonas sp. | |
1.2 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215V | Pseudomonas sp. | |
1.2 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y271F | Pseudomonas sp. | |
1.6 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, wild-type enzyme | Pseudomonas sp. | |
1.7 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215L | Pseudomonas sp. | |
1.7 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215Y | Pseudomonas sp. | |
1.7 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme D416Y | Pseudomonas sp. | |
1.7 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296S/H417M | Pseudomonas sp. | |
1.9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296F | Pseudomonas sp. | |
1.9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y27V | Pseudomonas sp. | |
2 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296T | Pseudomonas sp. | |
2 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y215Y/F270S | Pseudomonas sp. | |
2.5 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S | Pseudomonas sp. | |
2.7 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme F270M | Pseudomonas sp. | |
2.7 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme Y271V | Pseudomonas sp. | |
2.8 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296N | Pseudomonas sp. | |
3.2 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215E | Pseudomonas sp. | |
4 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme A215Y/H296S | Pseudomonas sp. | |
4.1 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296F | Pseudomonas sp. | |
4.3 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H417Y | Pseudomonas sp. | |
4.6 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H309S | Pseudomonas sp. | |
4.8 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296T | Pseudomonas sp. | |
5.2 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296N | Pseudomonas sp. | |
5.5 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296S/H417M | Pseudomonas sp. | |
6.3 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H309Y | Pseudomonas sp. | |
6.5 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme F270M | Pseudomonas sp. | |
6.7 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296S | Pseudomonas sp. | |
6.9 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215Y | Pseudomonas sp. | |
6.9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme H296S/H309S | Pseudomonas sp. | |
7.2 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme D416Y | Pseudomonas sp. | |
7.7 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215F | Pseudomonas sp. | |
8 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme Y271F | Pseudomonas sp. | |
8.3 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215V | Pseudomonas sp. | |
9 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme D416Y/H417Y | Pseudomonas sp. | |
9.5 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215L | Pseudomonas sp. | |
10 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215Y/H296S | Pseudomonas sp. | |
10.1 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme Y215Y/F270S | Pseudomonas sp. | |
11 | - |
cephalosporin C | pH 8.0, 37°C, wild-type enzyme | Pseudomonas sp. | |
12.2 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H296S/H309S | Pseudomonas sp. | |
12.3 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H417Y | Pseudomonas sp. | |
13 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme D416Y/H417Y | Pseudomonas sp. | |
17.2 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215Y/H309S | Pseudomonas sp. | |
17.8 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S | Pseudomonas sp. | |
18.9 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme E89A/A215Y | Pseudomonas sp. | |
24.4 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme H309S | Pseudomonas sp. | |
24.5 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y | Pseudomonas sp. | |
25 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y | Pseudomonas sp. | |
32 | - |
cephalosporin C | pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y | Pseudomonas sp. | |
37.2 | - |
glutaryl-7-amino cephalosporanic acid | pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
N176 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-amino cephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? |