Protein Variants | Comment | Organism |
---|---|---|
F375A | turnover number is 2.45fold lower than wild-type value, KM-value is 1.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375C | turnover number is 1.1fold higher than wild-type value, KM-value is 5.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 1.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375D | turnover number is 32.7fold lower than wild-type value, KM-value is 11fold higher than wild-type value, turnover number is 400fold lower than wild-type value, KM-value is 7fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375E | turnover number is 6.1fold lower than wild-type value, KM-value is 12fold higher than wild-type value, turnover number is 500fold lower than wild-type value, KM-value is 6.7fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375G | turnover number is 6.7fold lower than wild-type value, KM-value is 6.25fold lower than wild-type value, turnover number is 1.6fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375H | turnover number is 2.4fold higher than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 3fold lower than wild-type value, KM-value is 3.5fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375I | turnover number is 5fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 6fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375K | turnover number is 13.6fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value, turnover number is 1.8fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375L | turnover number is 1.4fold higher than wild-type value, KM-value is 1.4fold lower than wild-type value, turnover number is 3.6fold lower than wild-type value, KM-value is 8.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375M | turnover number is 1.3fold higher than wild-type value, KM-value is 1.5fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.5fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375N | turnover number is 1.8fold higher than wild-type value, KM-value is 1.2fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.8fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375Q | turnover number is 1.4fold higher than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 5fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375R | turnover number is 27.2fold lower than wild-type value, KM-value is 10fold higher than wild-type value, turnover number is 19fold lower than wild-type value, KM-value is 19fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375S | turnover number is 4.5fold lower than wild-type value, KM-value is nearly identical to wild-type value, turnover number is 1.3fold lower than wild-type value, KM-value is 2.3fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375T | turnover number is 2fold lower than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 2.2fold lower than wild-type value, KM-value is 2.9fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375V | turnover number is 5.4fold lower than wild-type value, KM-value is 1.4fold higher than wild-type value, turnover number is 3.9fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375W | turnover number is 18.8fold lower than wild-type value, KM-value is 1.5fold higher than wild-type value, turnover number is 8.5fold lower than wild-type value, KM-value is 25.8fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
F375Y | turnover number is 1.14fold lower than wild-type value, KM-value is 2.4fold lower than wild-type value, turnover number is 1.1fold lower than wild-type value, KM-value is 2.6fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. SY-77-1 | |
0.038 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375C | Pseudomonas sp. SY-77-1 | |
0.066 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375G | Pseudomonas sp. SY-77-1 | |
0.07 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375A | Pseudomonas sp. SY-77-1 | |
0.07 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375S | Pseudomonas sp. SY-77-1 | |
0.08 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Y | Pseudomonas sp. SY-77-1 | |
0.09 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375T | Pseudomonas sp. SY-77-1 | |
0.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375I | Pseudomonas sp. SY-77-1 | |
0.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375V | Pseudomonas sp. SY-77-1 | |
0.11 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375H | Pseudomonas sp. SY-77-1 | |
0.16 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375G | Pseudomonas sp. SY-77-1 | |
0.17 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375C | Pseudomonas sp. SY-77-1 | |
0.17 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375M | Pseudomonas sp. SY-77-1 | |
0.18 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375N | Pseudomonas sp. SY-77-1 | |
0.2 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375E | Pseudomonas sp. SY-77-1 | |
0.22 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375D | Pseudomonas sp. SY-77-1 | |
0.26 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. SY-77-1 | |
0.51 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375A | Pseudomonas sp. SY-77-1 | |
0.6 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375R | Pseudomonas sp. SY-77-1 | |
0.65 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375M | Pseudomonas sp. SY-77-1 | |
0.7 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375K | Pseudomonas sp. SY-77-1 | |
0.7 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. SY-77-1 | |
0.7 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Q | Pseudomonas sp. SY-77-1 | |
0.7 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Y | Pseudomonas sp. SY-77-1 | |
0.8 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375W | Pseudomonas sp. SY-77-1 | |
0.83 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375N | Pseudomonas sp. SY-77-1 | |
0.9 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375H | Pseudomonas sp. SY-77-1 | |
0.9 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375T | Pseudomonas sp. SY-77-1 | |
0.99 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375S | Pseudomonas sp. SY-77-1 | |
1 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. SY-77-1 | |
1.37 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375V | Pseudomonas sp. SY-77-1 | |
1.5 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375W | Pseudomonas sp. SY-77-1 | |
2.07 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375I | Pseudomonas sp. SY-77-1 | |
2.1 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Q | Pseudomonas sp. SY-77-1 | |
2.16 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375K | Pseudomonas sp. SY-77-1 | |
10 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375R | Pseudomonas sp. SY-77-1 | |
11 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375D | Pseudomonas sp. SY-77-1 | |
12 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375E | Pseudomonas sp. SY-77-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. SY-77-1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77-1 | 7-aminocephalosporanic acid + adipate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77-1 | 7-aminocephalosporanic acid + glutarate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375E | Pseudomonas sp. SY-77-1 | |
0.01 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375D | Pseudomonas sp. SY-77-1 | |
0.015 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375D | Pseudomonas sp. SY-77-1 | |
0.018 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375R | Pseudomonas sp. SY-77-1 | |
0.026 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375W | Pseudomonas sp. SY-77-1 | |
0.036 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375K | Pseudomonas sp. SY-77-1 | |
0.073 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375G | Pseudomonas sp. SY-77-1 | |
0.08 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375E | Pseudomonas sp. SY-77-1 | |
0.09 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375V | Pseudomonas sp. SY-77-1 | |
0.097 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375I | Pseudomonas sp. SY-77-1 | |
0.11 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375S | Pseudomonas sp. SY-77-1 | |
0.2 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375A | Pseudomonas sp. SY-77-1 | |
0.21 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375R | Pseudomonas sp. SY-77-1 | |
0.24 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375T | Pseudomonas sp. SY-77-1 | |
0.43 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Y | Pseudomonas sp. SY-77-1 | |
0.47 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375W | Pseudomonas sp. SY-77-1 | |
0.49 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. SY-77-1 | |
0.55 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375C | Pseudomonas sp. SY-77-1 | |
0.65 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375M | Pseudomonas sp. SY-77-1 | |
0.67 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375I | Pseudomonas sp. SY-77-1 | |
0.67 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. SY-77-1 | |
0.68 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Q | Pseudomonas sp. SY-77-1 | |
0.8 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Q | Pseudomonas sp. SY-77-1 | |
0.9 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375N | Pseudomonas sp. SY-77-1 | |
1.02 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375V | Pseudomonas sp. SY-77-1 | |
1.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. SY-77-1 | |
1.16 | - |
adipyl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375H | Pseudomonas sp. SY-77-1 | |
1.334 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375H | Pseudomonas sp. SY-77-1 | |
1.8 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375T | Pseudomonas sp. SY-77-1 | |
1.96 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375N | Pseudomonas sp. SY-77-1 | |
2.02 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375M | Pseudomonas sp. SY-77-1 | |
2.2 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375K | Pseudomonas sp. SY-77-1 | |
2.308 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375C | Pseudomonas sp. SY-77-1 | |
2.35 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375A | Pseudomonas sp. SY-77-1 | |
2.46 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375G | Pseudomonas sp. SY-77-1 | |
3.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375S | Pseudomonas sp. SY-77-1 | |
3.64 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375Y | Pseudomonas sp. SY-77-1 | |
4 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. SY-77-1 |