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Literature summary for 3.5.1.93 extracted from

  • Sio, C.F.; Otten, L.G.; Cool, R.H.; Quax, W.J.
    Analysis of a substrate specificity switch residue of cephalosporin acylase (2003), Biochem. Biophys. Res. Commun., 312, 755-760.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F375A turnover number is 2.45fold lower than wild-type value, KM-value is 1.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value Pseudomonas sp. SY-77-1
F375C turnover number is 1.1fold higher than wild-type value, KM-value is 5.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 1.2fold higher than wild-type value Pseudomonas sp. SY-77-1
F375D turnover number is 32.7fold lower than wild-type value, KM-value is 11fold higher than wild-type value, turnover number is 400fold lower than wild-type value, KM-value is 7fold higher than wild-type value Pseudomonas sp. SY-77-1
F375E turnover number is 6.1fold lower than wild-type value, KM-value is 12fold higher than wild-type value, turnover number is 500fold lower than wild-type value, KM-value is 6.7fold higher than wild-type value Pseudomonas sp. SY-77-1
F375G turnover number is 6.7fold lower than wild-type value, KM-value is 6.25fold lower than wild-type value, turnover number is 1.6fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value Pseudomonas sp. SY-77-1
F375H turnover number is 2.4fold higher than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 3fold lower than wild-type value, KM-value is 3.5fold higher than wild-type value Pseudomonas sp. SY-77-1
F375I turnover number is 5fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 6fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value Pseudomonas sp. SY-77-1
F375K turnover number is 13.6fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value, turnover number is 1.8fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value Pseudomonas sp. SY-77-1
F375L turnover number is 1.4fold higher than wild-type value, KM-value is 1.4fold lower than wild-type value, turnover number is 3.6fold lower than wild-type value, KM-value is 8.4fold higher than wild-type value Pseudomonas sp. SY-77-1
F375M turnover number is 1.3fold higher than wild-type value, KM-value is 1.5fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.5fold higher than wild-type value Pseudomonas sp. SY-77-1
F375N turnover number is 1.8fold higher than wild-type value, KM-value is 1.2fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.8fold higher than wild-type value Pseudomonas sp. SY-77-1
F375Q turnover number is 1.4fold higher than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 5fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value Pseudomonas sp. SY-77-1
F375R turnover number is 27.2fold lower than wild-type value, KM-value is 10fold higher than wild-type value, turnover number is 19fold lower than wild-type value, KM-value is 19fold higher than wild-type value Pseudomonas sp. SY-77-1
F375S turnover number is 4.5fold lower than wild-type value, KM-value is nearly identical to wild-type value, turnover number is 1.3fold lower than wild-type value, KM-value is 2.3fold higher than wild-type value Pseudomonas sp. SY-77-1
F375T turnover number is 2fold lower than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 2.2fold lower than wild-type value, KM-value is 2.9fold higher than wild-type value Pseudomonas sp. SY-77-1
F375V turnover number is 5.4fold lower than wild-type value, KM-value is 1.4fold higher than wild-type value, turnover number is 3.9fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value Pseudomonas sp. SY-77-1
F375W turnover number is 18.8fold lower than wild-type value, KM-value is 1.5fold higher than wild-type value, turnover number is 8.5fold lower than wild-type value, KM-value is 25.8fold higher than wild-type value Pseudomonas sp. SY-77-1
F375Y turnover number is 1.14fold lower than wild-type value, KM-value is 2.4fold lower than wild-type value, turnover number is 1.1fold lower than wild-type value, KM-value is 2.6fold higher than wild-type value Pseudomonas sp. SY-77-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, wild-type enzyme Pseudomonas sp. SY-77-1
0.038
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375C Pseudomonas sp. SY-77-1
0.066
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375G Pseudomonas sp. SY-77-1
0.07
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375A Pseudomonas sp. SY-77-1
0.07
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375S Pseudomonas sp. SY-77-1
0.08
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Y Pseudomonas sp. SY-77-1
0.09
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375T Pseudomonas sp. SY-77-1
0.1
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375I Pseudomonas sp. SY-77-1
0.1
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375V Pseudomonas sp. SY-77-1
0.11
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375H Pseudomonas sp. SY-77-1
0.16
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375G Pseudomonas sp. SY-77-1
0.17
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375C Pseudomonas sp. SY-77-1
0.17
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375M Pseudomonas sp. SY-77-1
0.18
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375N Pseudomonas sp. SY-77-1
0.2
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375E Pseudomonas sp. SY-77-1
0.22
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375D Pseudomonas sp. SY-77-1
0.26
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375L Pseudomonas sp. SY-77-1
0.51
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375A Pseudomonas sp. SY-77-1
0.6
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375R Pseudomonas sp. SY-77-1
0.65
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375M Pseudomonas sp. SY-77-1
0.7
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375K Pseudomonas sp. SY-77-1
0.7
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375L Pseudomonas sp. SY-77-1
0.7
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Q Pseudomonas sp. SY-77-1
0.7
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Y Pseudomonas sp. SY-77-1
0.8
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375W Pseudomonas sp. SY-77-1
0.83
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375N Pseudomonas sp. SY-77-1
0.9
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375H Pseudomonas sp. SY-77-1
0.9
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375T Pseudomonas sp. SY-77-1
0.99
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375S Pseudomonas sp. SY-77-1
1
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, wild-type enzyme Pseudomonas sp. SY-77-1
1.37
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375V Pseudomonas sp. SY-77-1
1.5
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375W Pseudomonas sp. SY-77-1
2.07
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375I Pseudomonas sp. SY-77-1
2.1
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Q Pseudomonas sp. SY-77-1
2.16
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375K Pseudomonas sp. SY-77-1
10
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375R Pseudomonas sp. SY-77-1
11
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375D Pseudomonas sp. SY-77-1
12
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375E Pseudomonas sp. SY-77-1

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. SY-77-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adipyl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. SY-77-1 7-aminocephalosporanic acid + adipate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. SY-77-1 7-aminocephalosporanic acid + glutarate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375E Pseudomonas sp. SY-77-1
0.01
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375D Pseudomonas sp. SY-77-1
0.015
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375D Pseudomonas sp. SY-77-1
0.018
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375R Pseudomonas sp. SY-77-1
0.026
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375W Pseudomonas sp. SY-77-1
0.036
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375K Pseudomonas sp. SY-77-1
0.073
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375G Pseudomonas sp. SY-77-1
0.08
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375E Pseudomonas sp. SY-77-1
0.09
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375V Pseudomonas sp. SY-77-1
0.097
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375I Pseudomonas sp. SY-77-1
0.11
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375S Pseudomonas sp. SY-77-1
0.2
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375A Pseudomonas sp. SY-77-1
0.21
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375R Pseudomonas sp. SY-77-1
0.24
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375T Pseudomonas sp. SY-77-1
0.43
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Y Pseudomonas sp. SY-77-1
0.47
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375W Pseudomonas sp. SY-77-1
0.49
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, wild-type enzyme Pseudomonas sp. SY-77-1
0.55
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375C Pseudomonas sp. SY-77-1
0.65
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375M Pseudomonas sp. SY-77-1
0.67
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375I Pseudomonas sp. SY-77-1
0.67
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375L Pseudomonas sp. SY-77-1
0.68
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Q Pseudomonas sp. SY-77-1
0.8
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Q Pseudomonas sp. SY-77-1
0.9
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375N Pseudomonas sp. SY-77-1
1.02
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375V Pseudomonas sp. SY-77-1
1.1
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375L Pseudomonas sp. SY-77-1
1.16
-
adipyl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375H Pseudomonas sp. SY-77-1
1.334
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375H Pseudomonas sp. SY-77-1
1.8
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375T Pseudomonas sp. SY-77-1
1.96
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375N Pseudomonas sp. SY-77-1
2.02
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375M Pseudomonas sp. SY-77-1
2.2
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375K Pseudomonas sp. SY-77-1
2.308
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375C Pseudomonas sp. SY-77-1
2.35
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375A Pseudomonas sp. SY-77-1
2.46
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375G Pseudomonas sp. SY-77-1
3.1
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375S Pseudomonas sp. SY-77-1
3.64
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, mutant enzyme F375Y Pseudomonas sp. SY-77-1
4
-
glutaryl-7-aminocephalosporanic acid pH 7.5, 37°C, wild-type enzyme Pseudomonas sp. SY-77-1