Literature summary for 3.5.1.91 extracted from

Matsumoto, M.; Hashimoto, Y.; Saitoh, Y.; Kumano, T.; Kobayashi, M.
Development of nitrilase promoter-derived inducible vectors for Streptomyces (2016), Biosci. Biotechnol. Biochem., 80, 1230-1237 .

Search for more literature for 3.5.1.91

Cloned(Commentary)

Cloned (Comment)	Organism
gene nfdA, recombinant expression of the enzyme from vectors pESH19cF-nfdA, pESH19kF-nfdA, and pESH19aF-nfdA in Escherichia coli strain Stbl2 and Streptomyces lividans strain TK24	Arthrobacter pascens

Protein Variants

Protein Variants	Comment	Organism
additional information	development of six inducible shuttle vectors, pESH19cF, pESH19cR, pESH19kF, pESH19kR, pESH19aF, and pESH19aR, for Streptomyces-Escherichia coli, evaluation of stability and activity in expression of different enzymes including the N-substituted formamide deformylase as model enzymes, overview. Catechol 2,3-dioxygenase, nitrilase, and N-substituted formamide deformylase encoding genes are expressed from the different vectors as reporter genes showing that pESH19cF, pESH19kF, and pESH19aF possess inducible expression ability in Streptomyces lividans strain TK24. The stability test shows that the pSH19-derived shuttle vectors are stable in Escherichia coli strain Stbl2 and Streptomyces lividans strain TK24. Streptomyces lividans strain TK24 is the better host	Arthrobacter pascens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent on	Arthrobacter pascens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-benzylformamide + H2O	Arthrobacter pascens	-	formate + benzylamine	-	?
N-benzylformamide + H2O	Arthrobacter pascens F164	-	formate + benzylamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter pascens	Q68AP4	-	-
Arthrobacter pascens F164	Q68AP4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-benzylformamide + H2O	-	Arthrobacter pascens	formate + benzylamine	-	?
N-benzylformamide + H2O	-	Arthrobacter pascens F164	formate + benzylamine	-	?

Synonyms

Synonyms	Comment	Organism
NfdA	-	Arthrobacter pascens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Arthrobacter pascens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Arthrobacter pascens

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Release 2023.1 (January 2023)