Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain ArcticExpress | Chlamydia trachomatis |
Protein Variants | Comment | Organism |
---|---|---|
F134C | the mutation alters the enzyme activity (nearly 2fold higher Km and increased kcat value as compared with the wild type enzyme) and affects protein stability | Chlamydia trachomatis |
F134C/R137S | the mutation alters the enzyme activity (nearly 10fold higher Km, increased kcat and 27% decrease in the kcat/Km value as compared with the wild type enzyme) and is 6.3fold more resistant towards inhibitor GM6001 as the wild type enzyme | Chlamydia trachomatis |
R137S | the mutation alters the enzyme activity (nearly 2fold higher Km and increased kcat value as compared with the wild type enzyme) and affects protein stability | Chlamydia trachomatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(R)-[2-(hydroxyaminocarbonyl)methyl]-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide | - |
Chlamydia trachomatis | |
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide | - |
Chlamydia trachomatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
N-formyl-Met-Ala-Ser | wild type enzyme, at pH 7.2 and 37°C | Chlamydia trachomatis | |
9.2 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C, at pH 7.2 and 37°C | Chlamydia trachomatis | |
9.3 | - |
N-formyl-Met-Ala-Ser | mutant enzyme R137S, at pH 7.2 and 37°C | Chlamydia trachomatis | |
48 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C/R137S, at pH 7.2 and 37°C | Chlamydia trachomatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia trachomatis | - |
- |
- |
Chlamydia trachomatis GR10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON affinity metal resin column chromatography | Chlamydia trachomatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-Met-Ala-Ser + H2O | - |
Chlamydia trachomatis | formate + Met-Ala-Ser | - |
? | |
N-formyl-Met-Ala-Ser + H2O | - |
Chlamydia trachomatis GR10 | formate + Met-Ala-Ser | - |
? |
Synonyms | Comment | Organism |
---|---|---|
- |
Chlamydia trachomatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
N-formyl-Met-Ala-Ser | wild type enzyme, at pH 7.2 and 37°C | Chlamydia trachomatis | |
19.6 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C, at pH 7.2 and 37°C | Chlamydia trachomatis | |
22.1 | - |
N-formyl-Met-Ala-Ser | mutant enzyme R137S, at pH 7.2 and 37°C | Chlamydia trachomatis | |
45.3 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C/R137S, at pH 7.2 and 37°C | Chlamydia trachomatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000148 | - |
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide | mutant enzyme R137S, at pH 7.2 and 37°C | Chlamydia trachomatis | |
0.00016 | - |
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide | wild type enzyme, at pH 7.2 and 37°C | Chlamydia trachomatis | |
0.001 | - |
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide | mutant enzyme F134C/R137S, at pH 7.2 and 37°C | Chlamydia trachomatis | |
143 | - |
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide | mutant enzyme F134C, at pH 7.2 and 37°C | Chlamydia trachomatis |
Organism | Comment | Expression |
---|---|---|
Chlamydia trachomatis | the GR10 mutation increases transcription from the chlamydial peptide deformylase promoter by stabilizing interactions between the C-terminal domain of the RNA polymerase alpha subunit and upstream DNA | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.948 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C/R137S, at pH 7.2 and 37°C | Chlamydia trachomatis | |
1.296 | - |
N-formyl-Met-Ala-Ser | wild type enzyme, at pH 7.2 and 37°C | Chlamydia trachomatis | |
2.143 | - |
N-formyl-Met-Ala-Ser | mutant enzyme F134C, at pH 7.2 and 37°C | Chlamydia trachomatis | |
2.387 | - |
N-formyl-Met-Ala-Ser | mutant enzyme R137S, at pH 7.2 and 37°C | Chlamydia trachomatis |