Application | Comment | Organism |
---|---|---|
medicine | possible target for new broad spectrum antimicrobial agents | Staphylococcus aureus |
medicine | possible target for new broad spectrum antimicrobial agents | Escherichia coli |
medicine | possible target for new broad spectrum antimicrobial agents | Geobacillus stearothermophilus |
medicine | possible target for new broad spectrum antimicrobial agents | Enterococcus faecalis |
medicine | possible target for new broad spectrum antimicrobial agents | Plasmodium falciparum |
medicine | possible target for new broad spectrum antimicrobial agents | Trypanosoma sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a C-terminal 21 amino acid truncated enzyme | Escherichia coli |
General Stability | Organism |
---|---|
enhanced in vitro stability by binding of Zn2+ | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | - |
Arabidopsis thaliana | |
actinonin | pseudo-peptide hydroxamate, potent inhibitor Kd: 0.3 nM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | sensitive to reactive oxygen | Escherichia coli | |
Fe3+ | more stable activity than with Fe2+ | Escherichia coli | |
Ni2+ | gives insensitivity to oxidation | Escherichia coli | |
Zn2+ | cruical for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | Arabidopsis thaliana | - |
formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Plasmodium falciparum | - |
formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Trypanosoma sp. | - |
formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Geobacillus stearothermophilus | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Enterococcus faecalis | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
no activity in Caenorhabditis elegans | - |
- |
- |
no activity in Saccharomyces cerevisiae | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Solanum lycopersicum | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Trypanosoma sp. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to intracellular reactive oxygen | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | - |
Arabidopsis thaliana | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | - |
Plasmodium falciparum | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | - |
Trypanosoma sp. | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Staphylococcus aureus | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Geobacillus stearothermophilus | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Enterococcus faecalis | formate + L-methionine-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |