BRENDA - Enzyme Database
show all sequences of 3.5.1.84

Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841

Esquirol, L.; Peat, T.S.; Wilding, M.; Lucent, D.; French, N.G.; Hartley, C.J.; Newman, J.; Scott, C.; PLoS One 13, e0192736 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Rhizobium leguminosarum bv. viciae
Crystallization (Commentary)
Crystallization
Organism
crystals suitable for X-ray analysis are grown using a combination of seeding, in situ proteolysis, formulation variation and additive screening. Structures of the enzyme, the enzyme with bound inhibitor and variants of the enzyme
Rhizobium leguminosarum bv. viciae
Engineering
Amino acid exchange
Commentary
Organism
C175S
the mutant enzyme binds biuret but is non-catalytic. The structure of the inactive C175S mutant enzyme with substrate bound in the active site reveals that an active site cysteine (Cys175), aspartic acid (Asp36) and lysine (Lys142) form a catalytic triad, which is consistent with biochemical studies of enzyme variants
Rhizobium leguminosarum bv. viciae
F41A
kcat/Km for buiret is 540fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41L
kcat/Km for buiret is 713fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41W
kcat/Km for buiret is 73fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41Y
kcat/Km for buiret is 139fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
K142A
kcat/Km for buiret is 297fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215A
kcat/Km for buiret is 419fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215E
kcat/Km for buiret is 1302fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215N
kcat/Km for buiret is 38fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
0.079
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
0.127
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
0.139
-
Biuret
pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
0.333
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L
Rhizobium leguminosarum bv. viciae
0.505
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.749
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
2.073
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
2.528
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme is involved in cyanuric acid mineralization
urea-1-carboxylate + NH3
-
-
?
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme is involved in cyanuric acid mineralization
urea-1-carboxylate + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhizobium leguminosarum bv. viciae
Q1M7F4
-
-
Rhizobium leguminosarum bv. viciae 3841
Q1M7F4
-
-
Purification (Commentary)
Commentary
Organism
-
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biuret + H2O
-
746233
Rhizobium leguminosarum bv. viciae
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
the enzyme is involved in cyanuric acid mineralization
746233
Rhizobium leguminosarum bv. viciae
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
-
746233
Rhizobium leguminosarum bv. viciae 3841
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
the enzyme is involved in cyanuric acid mineralization
746233
Rhizobium leguminosarum bv. viciae 3841
urea-1-carboxylate + NH3
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.07
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L; pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
0.14
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.19
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
0.26
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
0.29
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
0.74
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
0.81
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
11.9
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rhizobium leguminosarum bv. viciae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystals suitable for X-ray analysis are grown using a combination of seeding, in situ proteolysis, formulation variation and additive screening. Structures of the enzyme, the enzyme with bound inhibitor and variants of the enzyme
Rhizobium leguminosarum bv. viciae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C175S
the mutant enzyme binds biuret but is non-catalytic. The structure of the inactive C175S mutant enzyme with substrate bound in the active site reveals that an active site cysteine (Cys175), aspartic acid (Asp36) and lysine (Lys142) form a catalytic triad, which is consistent with biochemical studies of enzyme variants
Rhizobium leguminosarum bv. viciae
F41A
kcat/Km for buiret is 540fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41L
kcat/Km for buiret is 713fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41W
kcat/Km for buiret is 73fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
F41Y
kcat/Km for buiret is 139fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
K142A
kcat/Km for buiret is 297fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215A
kcat/Km for buiret is 419fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215E
kcat/Km for buiret is 1302fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
Q215N
kcat/Km for buiret is 38fold lower than the wild-type value
Rhizobium leguminosarum bv. viciae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
0.079
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
0.127
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
0.139
-
Biuret
pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
0.333
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L
Rhizobium leguminosarum bv. viciae
0.505
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.749
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
2.073
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
2.528
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme is involved in cyanuric acid mineralization
urea-1-carboxylate + NH3
-
-
?
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme is involved in cyanuric acid mineralization
urea-1-carboxylate + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biuret + H2O
-
746233
Rhizobium leguminosarum bv. viciae
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
the enzyme is involved in cyanuric acid mineralization
746233
Rhizobium leguminosarum bv. viciae
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
-
746233
Rhizobium leguminosarum bv. viciae 3841
urea-1-carboxylate + NH3
-
-
-
?
biuret + H2O
the enzyme is involved in cyanuric acid mineralization
746233
Rhizobium leguminosarum bv. viciae 3841
urea-1-carboxylate + NH3
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.07
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L; pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
0.14
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.19
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
0.26
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
0.29
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
0.74
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
0.81
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
11.9
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in cyanuric acid mineralization
Rhizobium leguminosarum bv. viciae
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in cyanuric acid mineralization
Rhizobium leguminosarum bv. viciae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.115
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
0.21
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L
Rhizobium leguminosarum bv. viciae
0.277
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.357
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
0.504
-
Biuret
pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
1.081
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
2.047
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
3.958
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
149.742
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.115
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215E
Rhizobium leguminosarum bv. viciae
0.21
-
Biuret
pH 8.5, 28°C, mutant enzyme F41L
Rhizobium leguminosarum bv. viciae
0.277
-
Biuret
pH 8.5, 28°C, mutant enzyme F41A
Rhizobium leguminosarum bv. viciae
0.357
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215A
Rhizobium leguminosarum bv. viciae
0.504
-
Biuret
pH 8.5, 28°C, mutant enzyme K142A
Rhizobium leguminosarum bv. viciae
1.081
-
Biuret
pH 8.5, 28°C, mutant enzyme F41Y
Rhizobium leguminosarum bv. viciae
2.047
-
Biuret
pH 8.5, 28°C, mutant enzyme F41W
Rhizobium leguminosarum bv. viciae
3.958
-
Biuret
pH 8.5, 28°C, mutant enzyme Q215N
Rhizobium leguminosarum bv. viciae
149.742
-
Biuret
pH 8.5, 28°C, wild-type enzyme
Rhizobium leguminosarum bv. viciae
Other publictions for EC 3.5.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746233
Esquirol
Structural and biochemical ch ...
Rhizobium leguminosarum bv. viciae 3841, Rhizobium leguminosarum bv. viciae
PLoS One
13
e0192736
2018
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9
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4
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8
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1
1
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9
9
718459
Cameron
-
New family of biuret hydrolase ...
Rhizobium leguminosarum bv. viciae, Rhizobium leguminosarum bv. viciae 3841
ACS Catal.
1
1075-1082
2011
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1
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1
1
1
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1
1
2
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2
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1
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5
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43
1
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1
1
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43
1
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1
1
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1
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1
1
246621
Karns
-
Genes encoding s-triazine degr ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99
J. Agric. Food Chem.
45
1017-1022
1997
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1
1
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4
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5
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4
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4
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246626
Cook
-
Biodegradation of s-triazine x ...
Gordonia rubripertincta, Gordonia rubripertincta DSM 10347 / NRRLB-15444R, Hormodendrum sp., Klebsiella pneumoniae, Klebsiella pneumoniae 90, Klebsiella pneumoniae 99, Penicillium spp., Pseudomonas sp., Sporothrix schenckii
FEMS Microbiol. Rev.
46
93-116
1987
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2
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6
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12
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6
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6
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6
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246620
Cook
Ring cleavage and degradative ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99, Pseudomonas sp.
Biochem. J.
231
25-30
1985
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3
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