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Literature summary for 3.5.1.81 extracted from

  • Hambardzumyan, A.; Mkhitaryan, A.; Paloyan, A.; Dadayan, S.
    Covalent immobilization of D-aminoacylase of strain Rhodococcus armeniensis AM6.1 and the characteristics of the biocatalyst (2017), Appl. Biochem. Microbiol., 53, 20-24 .
No PubMed abstract available
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Protein Variants

Protein Variants Comment Organism
additional information immobilization of D-aminoacylase of the strain Rhodococcus armeniensis AM6.1 is carried out on the silochrome C-80 with a yield of enzymatic activity of 20%. Aminated silochrome is activated with glutaraldehyde, free aldehyde groups are blocked. The temperature and pH optima, thermal stability, and dependence of thermal stability on pH for free and immobilized enzymes are determined, overview Rhodococcus armeniensis

General Stability

General Stability Organism
D-valine stablizes the partially purified and immobilized enzyme, while Zn2+ destabilizes it. N-acetyl-D-methionine and glycerol have no or poor effects Rhodococcus armeniensis

Organism

Organism UniProt Comment Textmining
Rhodococcus armeniensis
-
-
-
Rhodococcus armeniensis AM6.1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the enzyme is partially purified Rhodococcus armeniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-methionine + H2O
-
 Rhodococcus armeniensis acetate + D-methionine
-
 ?
N-acetyl-D-methionine + H2O
-
 Rhodococcus armeniensis AM6.1 acetate + D-methionine
-
 ?

Synonyms

Synonyms Comment Organism
D-Aminoacylase
-
 Rhodococcus armeniensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 both free and immobilized enzyme Rhodococcus armeniensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 35 20 min, the free partially purified enzyme is most stable at 20°C with 50% activity remaining at 35°C Rhodococcus armeniensis
20 40 20 min, the immobilized partially purified enzyme is most stable at 20°C with 50% activity remaining at 40°C Rhodococcus armeniensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 immobilized enzyme Rhodococcus armeniensis
7.8
-
 free enzyme Rhodococcus armeniensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 20 min, the immobilized partially purified enzyme is most stable at pH 8.5 with over 50% stability at pH 7.0-10.0 and above Rhodococcus armeniensis
8 10 20 min, the free partially purified enzyme is most stable at pH 9.0 with over 50% stability at pH 8.0-10.0 Rhodococcus armeniensis