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Literature summary for 3.5.1.81 extracted from

  • Hambardzumyan, A.; Mkhitaryan, A.; Paloyan, A.; Dadayan, S.; Saghyan, A.
    Catalytic properties of aminoacylase of strain Rhodococcus armeniensis AM6.1 (2016), Appl. Biochem. Microbiol., 52, 250-255 .
No PubMed abstract available
Search for more literature for 3.5.1.81

Inhibitors

Inhibitors Comment Organism Structure
acetate slight competitive inhibition Rhodococcus armeniensis
acetic acid competitive inhibition Rhodococcus armeniensis
N-acetyl-D-leucine substrate inhibition Rhodococcus armeniensis
N-Acetyl-DL-tyrosine substrate inhibition Rhodococcus armeniensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, linear regression analysis, overview Rhodococcus armeniensis
2
-
 N-acetyl-D-leucine pH 7.8, 30°C Rhodococcus armeniensis
2.4
-
 N-acetyl-D-phenylalanine pH 7.8, 30°C Rhodococcus armeniensis
2.5
-
 acetate pH 7.8, 30°C Rhodococcus armeniensis
2.8
-
 N-acetyl-D-valine pH 7.8, 30°C Rhodococcus armeniensis
4.7
-
 N-acetyl-D-methionine pH 7.8, 30°C Rhodococcus armeniensis
8.5
-
 N-Acetyl-DL-tyrosine pH 7.8, 30°C Rhodococcus armeniensis
27.3
-
 N-acetyl-D-alanine pH 7.8, 30°C Rhodococcus armeniensis
66.9
-
 N-acetyl-DL-oxyvaline pH 7.8, 30°C Rhodococcus armeniensis

Organism

Organism UniProt Comment Textmining
Rhodococcus armeniensis
-
-
-
Rhodococcus armeniensis AM6.1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 substrate: N-acetyl-L-valine, pH 7.8, 30°C Rhodococcus armeniensis
0.22
-
 substrate: N-acetyl-L-methionine, pH 7.8, 30°C Rhodococcus armeniensis
1.1
-
 substrate: N-acetyl-DL-arginine, pH 7.8, 30°C Rhodococcus armeniensis
1.32
-
 substrate: N-acetyl-DL-tryptophane, pH 7.8, 30°C Rhodococcus armeniensis
1.55
-
 substrate: N-acetyl-D-leucine, pH 7.8, 30°C Rhodococcus armeniensis
2.1
-
 substrate: N-acetyl-DL-valine, pH 7.8, 30°C Rhodococcus armeniensis
2.76
-
 substrate: N-acetyl-D-valine, pH 7.8, 30°C Rhodococcus armeniensis
6.51
-
 substrate: N-acetyl-D-phenylalanine, pH 7.8, 30°C Rhodococcus armeniensis
6.95
-
 substrate: N-acetyl-D-alanine, pH 7.8, 30°C Rhodococcus armeniensis
7.95
-
 substrate: N-acetyl-DL-tyrosine, pH 7.8, 30°C Rhodococcus armeniensis
11.9
-
 substrate: N-acetyl-DL-allylglycine, pH 7.8, 30°C Rhodococcus armeniensis
16.12
-
 substrate: N-acetyl-DL-oxyvaline, pH 7.8, 30°C Rhodococcus armeniensis
22.41
-
 substrate: N-acetyl-DL-methionine, pH 7.8, 30°C Rhodococcus armeniensis
22.63
-
 substrate: N-acetyl-D-methionine, pH 7.8, 30°C Rhodococcus armeniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information absolute stereospecificity to the D-stereoisomers of N-acetyl-amino acids. The enzyme does practically not react with acidic and hydrophilic N-acetyl-amino acids Rhodococcus armeniensis ?
-
 ?
additional information exhibits absolute stereospecificity to the D-stereoisomers of N-acetyl-amino acids. The enzyme is most active with N-acetyl-D-methionine, as well as with aromatic and hydrophobic N-acetylamino acids and interacts weakly with basic substrates. It is practically not active with acidic and hydrophilic N-acetyl-amino acids. Substrate specificity, overview. No activity with N-acetyl-L-valine, N-acetyl-DL-serine, N-acetyl-DL-threonine, N-acetyl-DL-aspartic acid, and N-acetyl-L-methionine. Only D-enantiomers are produced in the hydrolysis of the racemic substrates Rhodococcus armeniensis ?
-
-
additional information absolute stereospecificity to the D-stereoisomers of N-acetyl-amino acids. The enzyme does practically not react with acidic and hydrophilic N-acetyl-amino acids Rhodococcus armeniensis AM6.1 ?
-
 ?
additional information exhibits absolute stereospecificity to the D-stereoisomers of N-acetyl-amino acids. The enzyme is most active with N-acetyl-D-methionine, as well as with aromatic and hydrophobic N-acetylamino acids and interacts weakly with basic substrates. It is practically not active with acidic and hydrophilic N-acetyl-amino acids. Substrate specificity, overview. No activity with N-acetyl-L-valine, N-acetyl-DL-serine, N-acetyl-DL-threonine, N-acetyl-DL-aspartic acid, and N-acetyl-L-methionine. Only D-enantiomers are produced in the hydrolysis of the racemic substrates Rhodococcus armeniensis AM6.1 ?
-
-
N-acetyl-D-alanine + H2O low activity Rhodococcus armeniensis acetate + D-alanine
-
 r
N-acetyl-D-alanine + H2O activity is 30.7% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-alanine
-
 ?
N-acetyl-D-allylglycine + H2O moderate activity Rhodococcus armeniensis acetate + D-allylglycine
-
 r
N-acetyl-D-arginine + H2O low activity Rhodococcus armeniensis acetate + D-arginine
-
 r
N-acetyl-D-leucine + H2O low activity Rhodococcus armeniensis acetate + D-leucine
-
 r
N-acetyl-D-leucine + H2O activity is 6.8% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-leucine
-
 ?
N-acetyl-D-leucine + H2O low activity Rhodococcus armeniensis AM6.1 acetate + D-leucine
-
 r
N-acetyl-D-leucine + H2O activity is 6.8% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis AM6.1 acetate + D-leucine
-
 ?
N-acetyl-D-methionine + H2O
-
 Rhodococcus armeniensis acetate + D-methionine
-
 ?
N-acetyl-D-methionine + H2O high activity, best substrate Rhodococcus armeniensis acetate + D-methionine
-
 r
N-acetyl-D-methionine + H2O
-
 Rhodococcus armeniensis AM6.1 acetate + D-methionine
-
 ?
N-acetyl-D-oxyvaline + H2O activity is 71.2% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-oxyvaline
-
 ?
N-acetyl-D-phenylalanine + H2O low activity Rhodococcus armeniensis acetate + D-phenylalanine
-
 r
N-acetyl-D-phenylalanine + H2O activity is 28.8% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-phenylalanine
-
 ?
N-acetyl-D-tyrosine + H2O activity is 35.1% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-tyrosine
-
 ?
N-acetyl-D-valine + H2O low activity Rhodococcus armeniensis acetate + D-valine
-
 r
N-acetyl-D-valine + H2O activity is 9,3% compared to the activity with N-acetyl-D-methionine Rhodococcus armeniensis acetate + D-valine
-
 ?
N-acetyl-DL-methionine + H2O high activity Rhodococcus armeniensis acetate + D-methionine
-
 r
N-acetyl-DL-oxyvaline + H2O high activity Rhodococcus armeniensis acetate + D-oxyvaline
-
 r
N-acetyl-DL-tyrosine + H2O low activity Rhodococcus armeniensis acetate + D-tyrosine
-
 r
N-acetyl-DL-valine + H2O low activity Rhodococcus armeniensis acetate + D-valine
-
 r

Synonyms

Synonyms Comment Organism
D-Aminoacylase
-
 Rhodococcus armeniensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodococcus armeniensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Rhodococcus armeniensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
15.8
-
 N-Acetyl-DL-tyrosine pH 7.8, 30°C, substrate inhibition Rhodococcus armeniensis
35.5
-
 N-acetyl-D-leucine pH 7.8, 30°C, substrate inhibition Rhodococcus armeniensis
104.7
-
 acetate pH 7.8, 30°C Rhodococcus armeniensis
104.7
-
 acetic acid pH and temperature not specified in the publication Rhodococcus armeniensis