BRENDA - Enzyme Database
show all sequences of 3.5.1.7

Enzymatic synthesis and breakdown of a pyrimidine, orotic acid. III. ureidosuccinase

Lieberman, I.; Kornberg, A.; J. Biol. Chem. 212, 909-920 (1955)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cysteine
-
Faecalicatena orotica
glutathione
effect less than half that of cysteine
Faecalicatena orotica
sulfhydryl compound
-
Faecalicatena orotica
Inhibitors
Inhibitors
Commentary
Organism
Structure
Sodium thioglycolate
-
Faecalicatena orotica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Faecalicatena orotica
Fe2+
-
Faecalicatena orotica
Mn2+
absolute requirement
Faecalicatena orotica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ureidosuccinic acid + H2O
Faecalicatena orotica
only the L-isomer is attacked, the D form is completely inactive
?
-
-
-
additional information
Faecalicatena orotica
L-carboxymethylhydantoin is no substrate
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Faecalicatena orotica
-
-
-
Purification (Commentary)
Commentary
Organism
-
Faecalicatena orotica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4
-
-
Faecalicatena orotica
Storage Stability
Storage Stability
Organism
0°C stable for at least 10 days if kept in vacuo, loses activity rapidly when exposed to air either at 0°C or -16°C
Faecalicatena orotica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carbamoylglutamate + H2O
small amount of activity found
172076
Faecalicatena orotica
?
-
-
-
?
L-carboxymethylhydantoin + H2O
inert substrate
172076
Faecalicatena orotica
CO2 + ?
-
-
-
?
L-ureidosuccinic acid + H2O
only the L-isomer was attacked, the D form is completely inactive
172076
Faecalicatena orotica
L-aspartic acid + CO2 + NH3
-
-
-
?
L-ureidosuccinic acid + H2O
only the L-isomer is attacked, the D form is completely inactive
172076
Faecalicatena orotica
?
-
-
-
-
additional information
L-carboxymethylhydantoin is no substrate
172076
Faecalicatena orotica
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.5
-
Faecalicatena orotica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.1
9.6
-
Faecalicatena orotica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cysteine
-
Faecalicatena orotica
glutathione
effect less than half that of cysteine
Faecalicatena orotica
sulfhydryl compound
-
Faecalicatena orotica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Sodium thioglycolate
-
Faecalicatena orotica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
-
Faecalicatena orotica
Fe2+
-
Faecalicatena orotica
Mn2+
absolute requirement
Faecalicatena orotica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ureidosuccinic acid + H2O
Faecalicatena orotica
only the L-isomer is attacked, the D form is completely inactive
?
-
-
-
additional information
Faecalicatena orotica
L-carboxymethylhydantoin is no substrate
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Faecalicatena orotica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.4
-
-
Faecalicatena orotica
Storage Stability (protein specific)
Storage Stability
Organism
0°C stable for at least 10 days if kept in vacuo, loses activity rapidly when exposed to air either at 0°C or -16°C
Faecalicatena orotica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carbamoylglutamate + H2O
small amount of activity found
172076
Faecalicatena orotica
?
-
-
-
?
L-carboxymethylhydantoin + H2O
inert substrate
172076
Faecalicatena orotica
CO2 + ?
-
-
-
?
L-ureidosuccinic acid + H2O
only the L-isomer was attacked, the D form is completely inactive
172076
Faecalicatena orotica
L-aspartic acid + CO2 + NH3
-
-
-
?
L-ureidosuccinic acid + H2O
only the L-isomer is attacked, the D form is completely inactive
172076
Faecalicatena orotica
?
-
-
-
-
additional information
L-carboxymethylhydantoin is no substrate
172076
Faecalicatena orotica
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.5
-
Faecalicatena orotica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.1
9.6
-
Faecalicatena orotica
Other publictions for EC 3.5.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
172076
Lieberman
Enzymatic synthesis and breakd ...
Faecalicatena orotica
J. Biol. Chem.
212
909-920
1955
3
-
-
-
-
-
1
-
-
3
-
2
-
1
-
-
1
-
-
-
1
1
5
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
3
-
2
-
-
-
1
-
-
1
1
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-