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Literature summary for 3.5.1.69 extracted from

  • Huang, F.T.; Han, Y.B.; Feng, Y.; Yang, G.Y.
    A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production (2015), J. Lipid Res., 56, 1836-1842 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.69

Activating Compound

Activating Compound Comment Organism Structure
sodium cholate activates at 0.07-0.7% w/v, loss of about 75% activation potency in absence of Ca2+ Shewanella algae
taurodeoxycholate hydrate TDC, activates at 0.07-0.7% w/v, TDC-based enhancement of hydrolysis requires the presence of Ca2+. In the absence of Ca2+, GM1 hydrolysis is only 2.9%, 14fold lower than the same case of Triton X-100 Shewanella algae
Triton X-100 activates at 0.07-0.7% w/v, loss of about 50% activation potency in absence of Ca2+ Shewanella algae
Tween 80 activates 0.07-0.7% w/v, loss of about 65% activation potency in absence of Ca2+ Shewanella algae

Application

Application Comment Organism
synthesis sphingolipid ceramide N-deacylase is used for lyso-glycosphingolipid production. Application to large-scale preparation of gamglioside lyso-GM1, overview Shewanella algae

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Shewanella algae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, activtes. GM1 hydrolysis increases with increasing Ca 2+ concentration, compared with 40.7% yield in the absence of Ca2+, the addition of 100 mM Ca2+ enhances the yield to 81.6%. Best at 50-100 mM Shewanella algae
Co2+ activates, but slightly less effectve compared to Ca2+, at 100 mM Shewanella algae
Mg2+ activates, but less effectve compared to Ca2+, at 100 mM Shewanella algae
Mn2+ activates similar to Ca2+, at 100 mM Shewanella algae

Organism

Organism UniProt Comment Textmining
Shewanella algae Q8RTY6
-
-
Shewanella algae G8 Q8RTY6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) by nickel affinity chromatography Shewanella algae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactosylceramide + H2O
-
 Shewanella algae ?
-
 ?
ganglioside GM1 + H2O
-
 Shewanella algae ?
-
 ?
ganglioside GM1 + H2O
-
 Shewanella algae G8 ?
-
 ?
ganglioside GM3 + H2O
-
 Shewanella algae ?
-
 ?
ganglioside GM3 + H2O
-
 Shewanella algae G8 ?
-
 ?
glucosylceramide + H2O
-
 Shewanella algae ?
-
 ?
glucosylceramide + H2O
-
 Shewanella algae G8 ?
-
 ?
additional information assay method optimization and evaluation, overview Shewanella algae ?
-
 ?
additional information assay method optimization and evaluation, overview Shewanella algae G8 ?
-
 ?
sphingomyelin + H2O
-
 Shewanella algae ?
-
 ?
sphingomyelin + H2O
-
 Shewanella algae G8 ?
-
 ?

Synonyms

Synonyms Comment Organism
SA_SCD
-
 Shewanella algae
sphingolipid ceramide N-deacylase
-
 Shewanella algae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Shewanella algae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Shewanella algae

General Information

General Information Comment Organism
additional information development of a facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production Shewanella algae