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Literature summary for 3.5.1.69 extracted from

  • Hirabayashi, Y.; Kimura, M.; Matsumoto, M.; Yamamoto, K.; Kadowaki, S.; Tochikura, T.
    A novel glycosphingolipid hydrolyzing enzyme, glycosphingolipid ceramide deacylase, which cleaves the linkage between the fatty acid and sphingosine base in glycosphingolipids (1988), J. Biochem., 103, 1-4.
    View publication on PubMed
Search for more literature for 3.5.1.69

Activating Compound

Activating Compound Comment Organism Structure
sodium taurodeoxycholate 1.0 mg/ml, slight activation Nocardia sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, strong Nocardia sp.
EDTA 10 mM, 30% inhibition Nocardia sp.
Hg2+ 10 mM, strong Nocardia sp.
Mn2+ 10 mM, strong Nocardia sp.
Sn2+ 10 mM, strong Nocardia sp.
Zn2+ 10 mM, strong Nocardia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane tightly bound to Nocardia sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, slight activation Nocardia sp.
Mg2+ 10 mM, slight activation Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ganglioside asialo-GM1 + H2O
-
 Nocardia sp. ?
-
 ?
ganglioside asialo-GM2 + H2O
-
 Nocardia sp. ?
-
 ?
ganglioside Gb3-ceramide + H2O
-
 Nocardia sp. ?
-
 ?
ganglioside GD1a + H2O
-
 Nocardia sp. fatty acid + lyso-GD1a-ganglioside
-
 ?
ganglioside GM1 + H2O
-
 Nocardia sp. octadecanoic acid + lyso-GM1-ganglioside
-
 ?
ganglioside GM2 + H2O
-
 Nocardia sp. fatty acid + lyso-GM2-ganglioside
-
 ?
ganglioside GM3 + H2O
-
 Nocardia sp. fatty acid + lyso-GM3-ganglioside
-
 ?
additional information does not act on sphingolipids such as ceramide, Galbeta(1-1')ceramide, Glcbeta(1-1')ceramide, Galbeta(1-4)Glcbeta(1-1')ceramide Nocardia sp. ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 7.2, 30 min, stable Nocardia sp.
55
-
 pH 7.2, 30 min, 50% loss of activity Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Nocardia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2
-
 45°C, 30 min, stable Nocardia sp.