BRENDA - Enzyme Database show
show all sequences of 3.5.1.61

Isolation of a mimosine degrading enzyme from Leucaena leaf

Tangendjaja, B.; Lowry, J.B.; Wills, R.B.H.; J. Sci. Food Agric. 37, 523-526 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
3-hydroxy-4(1H)-pyridone, DHP, 0.4-3.2 mM, no effect, no feedback inhibitor
Leucaena leucocephala
tyrosine
competitive inhibitor, 0.14 mM: 90% of maximal activity, 0.28 mM: 80% of maximal activity, 0.42 mM: 78% of maximal activity, 0.56 mM: 72% of maximal activity, 0.96 mM: 56% of maximal activity, 1 mM: 50% of maximal activity
Leucaena leucocephala
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
reaction kinetics
Leucaena leucocephala
1.64
-
beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid
mimosine
Leucaena leucocephala
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
PAGE
Leucaena leucocephala
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leucaena leucocephala
-
tropical legume
-
Purification (Commentary)
Commentary
Organism
partial
Leucaena leucocephala
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Leucaena leucocephala
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
free toxic amino acid mimosine, 3-(3-hydroxy-4-oxo-1(4H)-pyridyl)-L-alanine, beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid, beta-[N-(3-hydroxy-4-oxypyridyl)]-alpha-aminopropionic acid
209216
Leucaena leucocephala
3-hydroxy-4(1H)-pyrid-4-one + L-serine
DHP, less toxic than mimosine
209216
Leucaena leucocephala
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Leucaena leucocephala
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Leucaena leucocephala
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
3-hydroxy-4(1H)-pyridone, DHP, 0.4-3.2 mM, no effect, no feedback inhibitor
Leucaena leucocephala
tyrosine
competitive inhibitor, 0.14 mM: 90% of maximal activity, 0.28 mM: 80% of maximal activity, 0.42 mM: 78% of maximal activity, 0.56 mM: 72% of maximal activity, 0.96 mM: 56% of maximal activity, 1 mM: 50% of maximal activity
Leucaena leucocephala
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
reaction kinetics
Leucaena leucocephala
1.64
-
beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid
mimosine
Leucaena leucocephala
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
PAGE
Leucaena leucocephala
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Leucaena leucocephala
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Leucaena leucocephala
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
free toxic amino acid mimosine, 3-(3-hydroxy-4-oxo-1(4H)-pyridyl)-L-alanine, beta-[N-(3-hydroxy-4(1H)-pyridone)]-alpha-aminopropionic acid, beta-[N-(3-hydroxy-4-oxypyridyl)]-alpha-aminopropionic acid
209216
Leucaena leucocephala
3-hydroxy-4(1H)-pyrid-4-one + L-serine
DHP, less toxic than mimosine
209216
Leucaena leucocephala
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Leucaena leucocephala
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Leucaena leucocephala
Other publictions for EC 3.5.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734969
Negi
A carbon-nitrogen lyase from L ...
Leucaena leucocephala
Plant Physiol.
164
922-934
2014
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1
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-
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2
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1
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1
1
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3
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1
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3
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2
1
1
1
2
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1
1
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2
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1
2
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2
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1
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1
1
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1
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3
-
-
2
1
1
1
2
-
1
1
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-
-
1
1
-
-
-
209216
Tangendjaja
-
Isolation of a mimosine degrad ...
Leucaena leucocephala
J. Sci. Food Agric.
37
523-526
1986
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2
2
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1
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1
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1
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1
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1
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1
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1
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2
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2
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1
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1
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1
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1
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1
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1
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-
-
-
-
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209217
Tangendjaja
-
Optimisation of conditions for ...
Leucaena leucocephala
J. Sci. Food Agric.
35
613-616
1984
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1
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-
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1
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1
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1
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1
1
1
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1
1
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1
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1
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1
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1
1
1
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1
1
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