Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is proteolytically activated by an autocatalytic step under acidic conditions where the polypeptide is cleaved into two chains | Homo sapiens |
Application | Comment | Organism |
---|---|---|
pharmacology | potential of blocking N-acylethanolamines like palmitoylethanolamide or N-arachidonlyethanolamine (anandamide) from enzymatic degradation via enzyme inhibition as a strategy for pain treatment | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant stable expression of the His6-tagged zymogen in HEK-293 cells, ammonium chloride treatment stimulates secretion of the lysosomal proteins from the HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | selective alkylation of the enzyme's cysteine residues results in almost complete loss of activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | selective alkylation of the enzyme's cysteine residues results in almost complete loss of activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.0062 | - |
N-(4-methylcoumarin)palmitamide | pH 4.5, 37°C | Homo sapiens | |
0.021 | - |
palmitoylethanolamide | pH 4.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | the enzyme is believed to be transported by the mannose-6-phosphate pathway to the acidic late endosomes and/or lysosomes | Homo sapiens | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10972 | - |
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE | Homo sapiens |
14600 | - |
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE | Homo sapiens |
29659 | - |
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE | Homo sapiens |
33000 | - |
1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE | Homo sapiens |
40593 | - |
1 * 40593, deglycosylated zymogen, mass spectrometry | Homo sapiens |
45000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q02083 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 4 glycosylation sites Asn37, Asn107, Asn309, and Asn333, N-linked oligosaccharides of approximately 1500 Da, all cleavable by peptide-N-glycosidase F | Homo sapiens |
proteolytic modification | the glycoprotein undergoes removal of an N-terminal signal peptide after biosynthesis. Autocatalytic acid cleavage of the zymogen into alpha- and beta-subunits (14.6 and 33.3 kDa) activates the enzyme. Cys126 is essential for the proteolytic cleavage of the pro-enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged zymogen from HEK-293 cells by ammonium sulfate fractionation, dialysis, metal affinity chromatography, and again dialysis followed by ultrafiltration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(4-methylcoumarin)palmitamide + H2O | - |
Homo sapiens | palmitic acid + 7-amino-4-methylcoumarin | - |
? | |
palmitoylethanolamide + H2O | - |
Homo sapiens | palmitic acid + ethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 10972, deglycosylated alpha-subunit, + 1 * 29659, deglycosylated beta-subunit, mass spectrometry, 1 * 14600 + 1 * 33000, alpha/beta heterodimer, SDS-PAGE | Homo sapiens |
monomer | 1 * 40593, deglycosylated zymogen, mass spectrometry | Homo sapiens |
More | tryptic digestion and MALDI-TOF mass spectrometry fingerprinting gives evidence for the lack of a disulfide bond between subunits | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
N-acylethanolamine-hydrolyzing acid amidase | - |
Homo sapiens |
NAAA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the catalytic triad, is formed by cysteine, aspartate and arginine | Homo sapiens |
physiological function | N-acylethanolamine-hydrolyzing acid amidase is a lysosomal enzyme that primarily degrades palmitoylethanolamine, a lipid amide that inhibits inflammatory responses | Homo sapiens |