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Literature summary for 3.5.1.60 extracted from

  • Schmid, P.C.; Zuzarte-Augustin, M.L.; Schmid, H.H.O.
    Properties of rat liver N-acylethanolamine amidohydrolase (1985), J. Biol. Chem., 260, 14145-14149.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 0.5 mM: 50% inhibition of mitochondrial, 42% inhibition of microsomal enzyme Rattus norvegicus
N-ethylmaleimide 0.25 mM: 50% inhibition of mitochondrial enzyme, 0.5 mM: 50% inhibition of microsomal enzyme Rattus norvegicus
oleic acid 10 nM: 50% inhibition, 100 nM: 95% inhibition Rattus norvegicus
p-chloromercuribenzoate 0.05 mM: total inhibition of mitochondrial or microsomal enzyme Rattus norvegicus
sodium cholate 7.5 mg/ml: 90% inhibition Rattus norvegicus
sodium dodecylsulfate 2.5 mg/ml: 98% inhibition Rattus norvegicus
sodium taurodeoxycholate 15 mg/ml: 20% inhibition Rattus norvegicus
Triton X-100 5 mg/ml: 42% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025 0.05 N-oleoylethanolamine mitochondrial enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-40°C, solubilized enzyme, stable for several weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-acylsphingosine or N,O-diacylethanolamine are no substrates Rattus norvegicus ?
-
?
N-dodecanoylethanolamine + H2O 110% of activity compared to N-oleoylethanolamine Rattus norvegicus dodecanoic acid + ethanolamine
-
?
N-hexadecanoylethanolamine + H2O 71% of activity compared to N-oleoylethanolamine Rattus norvegicus hexadecanoic acid + ethanolamine
-
?
N-octadecanoylethanolamine + H2O 42% of activity compared to N-oleoylethanolamine Rattus norvegicus octadecanoic acid + ethanolamine
-
?
N-oleoylethanolamine + H2O best substrate in mixture of N-acylethanolamines Rattus norvegicus oleic acid + ethanolamine
-
r
N-tetradecanoylethanolamine + H2O 95% of activity compared to N-oleoylethanolamine Rattus norvegicus tetradecanoic acid + ethanolamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Rattus norvegicus
10.5
-
reverse reaction Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
oleic acid
-
Rattus norvegicus
0.5
-
5,5'-dithiobis(2-nitrobenzoic acid) mitochondrial Rattus norvegicus
25
-
N-ethylmaleimide mitochondrial enzyme Rattus norvegicus