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Literature summary for 3.5.1.49 extracted from

  • Borges, C.L.; Parente, J.A.; Barbosa, M.S.; Santana, J.M.; Bao, S.N.; de Sousa, M.V.; de Almeida Soares, C.M.
    Detection of a homotetrameric structure and protein-protein interactions of Paracoccidioides brasiliensis formamidase lead to new functional insights (2010), FEMS Yeast Res., 10, 104-113.
    View publication on PubMed
Search for more literature for 3.5.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paracoccidioides brasiliensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Paracoccidioides brasiliensis 5618
-
cytoplasm
-
 Paracoccidioides brasiliensis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
 4 * 43300, native state, native PAGE Paracoccidioides brasiliensis
45000
-
 4 * 45000, SDS-PAGE Paracoccidioides brasiliensis
180000
-
 SDS-PAGE, the 180000 Da purified protein yields a heat-denatured species of 45000 Da Paracoccidioides brasiliensis
191000
-
 purified formamidase, native PAGE Paracoccidioides brasiliensis

Organism

Organism UniProt Comment Textmining
Paracoccidioides brasiliensis C1GWV9 isolate 01 (ATCC MYA-826)
-

Purification (Commentary)

Purification (Comment) Organism
glutatione Sepharose column chromatography, DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography Paracoccidioides brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formamide + H2O
-
 Paracoccidioides brasiliensis formate + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 45000, SDS-PAGE Paracoccidioides brasiliensis
homotetramer 4 * 43300, native state, native PAGE Paracoccidioides brasiliensis

Synonyms

Synonyms Comment Organism
Fmd
-
 Paracoccidioides brasiliensis
formamide aminohydrolase
-
 Paracoccidioides brasiliensis