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Literature summary for 3.5.1.41 extracted from

  • Araki, Y.; Ito, E.
    A pathway of chitosan formation in Mucor rouxii. Enzymatic deacetylation of chitin (1975), Eur. J. Biochem., 55, 71-78.
    View publication on PubMed
Search for more literature for 3.5.1.41

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Amylomyces rouxii

General Stability

General Stability Organism
freezing in presence of ammonium sulfate causes inactivation, dialysis before storage Amylomyces rouxii

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
 Amylomyces rouxii
Co2+
-
 Amylomyces rouxii
EDTA
-
 Amylomyces rouxii
glycol chitosan
-
 Amylomyces rouxii
Mn2+
-
 Amylomyces rouxii
Na+
-
 Amylomyces rouxii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
 glycol chitin
-
 Amylomyces rouxii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Amylomyces rouxii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Amylomyces rouxii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Staphylococcus aureus
-
 chitosan + acetate
-
 ?
chitin + H2O Bacillus subtilis
-
 chitosan + acetate
-
 ?
chitin + H2O Escherichia coli
-
 chitosan + acetate
-
 ?
chitin + H2O Aspergillus niger
-
 chitosan + acetate
-
 ?
chitin + H2O Priestia megaterium
-
 chitosan + acetate
-
 ?
chitin + H2O Enterococcus faecalis
-
 chitosan + acetate
-
 ?
chitin + H2O Bacillus cereus
-
 chitosan + acetate
-
 ?
chitin + H2O Aspergillus oryzae
-
 chitosan + acetate
-
 ?
chitin + H2O Aspergillus parasiticus
-
 chitosan + acetate
-
 ?
chitin + H2O Amylomyces rouxii
-
 chitosan + acetate
-
 ?
chitin + H2O Bacillus subtilis AHU 1031
-
 chitosan + acetate
-
 ?
chitin + H2O Amylomyces rouxii AHU 6019
-
 chitosan + acetate
-
 ?
chitin + H2O Bacillus subtilis IAM 1069
-
 chitosan + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Amylomyces rouxii
-
 AHU 6019
-
Amylomyces rouxii AHU 6019
-
 AHU 6019
-
Aspergillus niger
-
 low enzyme level
-
Aspergillus oryzae
-
 low enzyme level
-
Aspergillus parasiticus
-
 low enzyme level
-
Bacillus cereus
-
 low enzyme level
-
Bacillus subtilis
-
 low enzyme level
-
Bacillus subtilis
-
 AHU 1031
-
Bacillus subtilis
-
 IAM 1069
-
Bacillus subtilis AHU 1031
-
 AHU 1031
-
Bacillus subtilis IAM 1069
-
 IAM 1069
-
Enterococcus faecalis
-
 low enzyme level
-
Escherichia coli
-
 low enzyme level
-
Priestia megaterium
-
 low enzyme level
-
Staphylococcus aureus
-
 low enzyme level
-

Purification (Commentary)

Purification (Comment) Organism
140fold, partial Amylomyces rouxii

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Amylomyces rouxii
-
mycelium
-
 Amylomyces rouxii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.8
-
-
 Amylomyces rouxii

Storage Stability

Storage Stability Organism
-18°C, stable for several months Amylomyces rouxii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Staphylococcus aureus chitosan + acetate
-
 ?
chitin + H2O
-
 Bacillus subtilis chitosan + acetate
-
 ?
chitin + H2O
-
 Escherichia coli chitosan + acetate
-
 ?
chitin + H2O
-
 Aspergillus niger chitosan + acetate
-
 ?
chitin + H2O
-
 Priestia megaterium chitosan + acetate
-
 ?
chitin + H2O
-
 Enterococcus faecalis chitosan + acetate
-
 ?
chitin + H2O
-
 Bacillus cereus chitosan + acetate
-
 ?
chitin + H2O
-
 Aspergillus oryzae chitosan + acetate
-
 ?
chitin + H2O
-
 Aspergillus parasiticus chitosan + acetate
-
 ?
chitin + H2O
-
 Amylomyces rouxii chitosan + acetate
-
 ?
chitin + H2O colloidal chitin Amylomyces rouxii chitosan + acetate
-
 ?
chitin + H2O powdered chitin, poor Amylomyces rouxii chitosan + acetate
-
 ?
chitin + H2O
-
 Bacillus subtilis AHU 1031 chitosan + acetate
-
 ?
chitin + H2O
-
 Amylomyces rouxii AHU 6019 chitosan + acetate
-
 ?
chitin + H2O colloidal chitin Amylomyces rouxii AHU 6019 chitosan + acetate
-
 ?
chitin + H2O powdered chitin, poor Amylomyces rouxii AHU 6019 chitosan + acetate
-
 ?
chitin + H2O
-
 Bacillus subtilis IAM 1069 chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Staphylococcus aureus chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Bacillus subtilis chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Escherichia coli chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Aspergillus niger chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Priestia megaterium chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Enterococcus faecalis chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Bacillus cereus chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Aspergillus oryzae chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Aspergillus parasiticus chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Amylomyces rouxii chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Bacillus subtilis AHU 1031 chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Amylomyces rouxii AHU 6019 chitosan + acetate
-
 ?
glycol chitin + H2O
-
 Bacillus subtilis IAM 1069 chitosan + acetate
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Amylomyces rouxii ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Amylomyces rouxii ?
-
 ?
N,N',N''-triacetylchitotriose + H2O poor Amylomyces rouxii ?
-
 ?
N,N',N''-triacetylchitotriose + H2O poor Amylomyces rouxii AHU 6019 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Amylomyces rouxii

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Amylomyces rouxii