BRENDA - Enzyme Database
show all sequences of 3.5.1.40

Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides

Takahashi, Y.; Shirai, T.; Ishii, S.; J. Biochem. 77, 823-830 (1975)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
84% inhibition at 50 mM
Fusarium anguioides
benzoyl-L-Arg-ethylester
Ki: 3.6 mM
Fusarium anguioides
benzoyl-L-Arg-NH2
Ki: 5 mM
Fusarium anguioides
Co2+
74% inhibition at 10 mM
Fusarium anguioides
Ni2+
85% inhibition at 10 mM
Fusarium anguioides
p-chloromercuribenzoic acid
77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Fusarium anguioides
Zn2+
95% inhibition at 10 mM
Fusarium anguioides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
gel filtration
Fusarium anguioides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusarium anguioides
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Fusarium anguioides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7
-
-
Fusarium anguioides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bleomycin B2 + H2O
specific with respect to the alkyl-chain length of the substrate
172037
Fusarium anguioides
bleomycinic acid + agmatine
-
172037
Fusarium anguioides
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Fusarium anguioides
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6
-
benzoyl-L-Arg-ethylester
-
Fusarium anguioides
5
-
benzoyl-L-Arg-NH2
-
Fusarium anguioides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
84% inhibition at 50 mM
Fusarium anguioides
benzoyl-L-Arg-ethylester
Ki: 3.6 mM
Fusarium anguioides
benzoyl-L-Arg-NH2
Ki: 5 mM
Fusarium anguioides
Co2+
74% inhibition at 10 mM
Fusarium anguioides
Ni2+
85% inhibition at 10 mM
Fusarium anguioides
p-chloromercuribenzoic acid
77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Fusarium anguioides
Zn2+
95% inhibition at 10 mM
Fusarium anguioides
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6
-
benzoyl-L-Arg-ethylester
-
Fusarium anguioides
5
-
benzoyl-L-Arg-NH2
-
Fusarium anguioides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
gel filtration
Fusarium anguioides
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Fusarium anguioides
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Fusarium anguioides
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7
-
-
Fusarium anguioides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bleomycin B2 + H2O
specific with respect to the alkyl-chain length of the substrate
172037
Fusarium anguioides
bleomycinic acid + agmatine
-
172037
Fusarium anguioides
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Fusarium anguioides
Other publictions for EC 3.5.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
172037
Takahashi
Characterizations of acylagmat ...
Fusarium anguioides
J. Biochem.
77
823-830
1975
-
-
-
-
-
-
7
-
-
-
1
-
-
3
-
-
1
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
7
2
-
-
-
1
-
-
-
-
1
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
172038
Umezawa
Preparation of bleomycinic aci ...
Fusarium anguioides
J. Antibiot.
26
117-119
1973
-
-
-
-
-
-
-
4
1
-
-
-
-
2
-
-
1
-
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
1
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-