BRENDA - Enzyme Database show
show all sequences of 3.5.1.36

5-Hydroxy-N-methylpyroglutamate synthase. Purification and mechanism of action

Hersh, L.B.; J. Biol. Chem. 245, 3526-3535 (1970)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-hydroxy-4-methoxy-benzophenone
with 1 micromol 2-hydroxy-4-methoxy-benzophenone the activity is 0% after 10 min
Pseudomonas sp.
DTNB
with 20 micromol DTNB the activity is 5% after 10 min
Pseudomonas sp.
iodoacetamide
with 20 micromol iodoacetamide the activity is 20% after 10 min
Pseudomonas sp.
iodoacetate
with 20 micromol iodoacetate the activity is 33% after 10 min
Pseudomonas sp.
N-Methylmaleimide
with 20 micromol N-methylmaleimide the activity is 2% after 10 min
Pseudomonas sp.
NEM
with 20 micromol NEM the activity is 0% after 10 min
Pseudomonas sp.
oxalacetate
a competitive inhibitor
Pseudomonas sp.
p-hydroxymercurisulfonate
with 1 micromol p-hydroxymercurisulfonate the activity is 0% after 10 min
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
alpha-ketoglutaramate
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-ketoglutaramate + H2O
Pseudomonas sp.
-
alpha-ketoglutarate + NH3
-
Pseudomonas sp.
?
alpha-ketoglutaramate + H2O
Pseudomonas sp. MA / M.A. / ATCC 23819
-
alpha-ketoglutarate + NH3
-
Pseudomonas sp. MA / M.A. / ATCC 23819
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-
Purification (Commentary)
Commentary
Organism
extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11
-
after purification
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.4, containing 5 mM alpha-ketoglutarate, six months. In the absence of alpha-ketoglutarate the enzyme slowly loses activity over a period of several weeks
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ketoglutaramate + H2O
-
209087
Pseudomonas sp.
alpha-ketoglutarate + NH3
-
209087
Pseudomonas sp.
?
alpha-ketoglutaramate + H2O
-
209087
Pseudomonas sp. MA / M.A. / ATCC 23819
alpha-ketoglutarate + NH3
-
209087
Pseudomonas sp. MA / M.A. / ATCC 23819
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0178
-
alpha-ketoglutarate
turnover number based on the molecular weight of 90000
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-hydroxy-4-methoxy-benzophenone
with 1 micromol 2-hydroxy-4-methoxy-benzophenone the activity is 0% after 10 min
Pseudomonas sp.
DTNB
with 20 micromol DTNB the activity is 5% after 10 min
Pseudomonas sp.
iodoacetamide
with 20 micromol iodoacetamide the activity is 20% after 10 min
Pseudomonas sp.
iodoacetate
with 20 micromol iodoacetate the activity is 33% after 10 min
Pseudomonas sp.
N-Methylmaleimide
with 20 micromol N-methylmaleimide the activity is 2% after 10 min
Pseudomonas sp.
NEM
with 20 micromol NEM the activity is 0% after 10 min
Pseudomonas sp.
oxalacetate
a competitive inhibitor
Pseudomonas sp.
p-hydroxymercurisulfonate
with 1 micromol p-hydroxymercurisulfonate the activity is 0% after 10 min
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
alpha-ketoglutaramate
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-ketoglutaramate + H2O
Pseudomonas sp.
-
alpha-ketoglutarate + NH3
-
Pseudomonas sp.
?
alpha-ketoglutaramate + H2O
Pseudomonas sp. MA / M.A. / ATCC 23819
-
alpha-ketoglutarate + NH3
-
Pseudomonas sp. MA / M.A. / ATCC 23819
?
Purification (Commentary) (protein specific)
Commentary
Organism
extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11
-
after purification
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.4, containing 5 mM alpha-ketoglutarate, six months. In the absence of alpha-ketoglutarate the enzyme slowly loses activity over a period of several weeks
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ketoglutaramate + H2O
-
209087
Pseudomonas sp.
alpha-ketoglutarate + NH3
-
209087
Pseudomonas sp.
?
alpha-ketoglutaramate + H2O
-
209087
Pseudomonas sp. MA / M.A. / ATCC 23819
alpha-ketoglutarate + NH3
-
209087
Pseudomonas sp. MA / M.A. / ATCC 23819
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0178
-
alpha-ketoglutarate
turnover number based on the molecular weight of 90000
Pseudomonas sp.
Other publictions for EC 3.5.1.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209085
Hersh
The effect of aliphatic alcoho ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
J. Biol. Chem.
246
7804-7809
1971
5
-
-
-
-
-
4
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209086
Hersh
5-Hydroxy-N-Methylpyroglutamat ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
J. Biol. Chem.
246
6803-6806
1971
-
-
-
-
-
-
-
3
-
-
-
6
-
9
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209087
Hersh
5-Hydroxy-N-methylpyroglutamat ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
J. Biol. Chem.
245
3526-3535
1970
-
-
-
-
-
-
8
1
-
-
1
2
-
9
-
-
1
-
-
-
1
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
-
1
2
-
-
-
1
-
-
1
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
209088
Hersh
The enzymatic synthesis of 5-h ...
Pseudomonas sp., Pseudomonas sp. MA / M.A. / ATCC 23819
J. Biol. Chem.
244
4677-4683
1969
-
-
-
-
-
-
2
2
-
-
-
2
-
9
-
-
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-