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Literature summary for 3.5.1.29 extracted from

  • Yuan, B.; Yokochi, N.; Yoshikane, Y.; Ohnishi, K.; Ge, F.; Yagi, T.
    Gene identification and characterization of the pyridoxine degradative enzyme alpha-(N-acetylaminomethylene)succinic acid amidohydrolase from Mesorhizobium loti MAFF303099 (2008), J. Nutr. Sci. Vitaminol., 54, 185-190.
    View publication on PubMed
Search for more literature for 3.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) and JM109 cells Mesorhizobium loti

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by Zn2+, Fe2+, or Ni2+ (1 mM each), the enzyme is not affected when incubated for 30 min at 30°C in buffer containing 100 mM EDTA Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0537
-
 alpha-(N-acetylaminomethylene)succinic acid at 25°C Mesorhizobium loti

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by Zn2+, Fe2+, or Ni2+ (1 mM each) Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29896
-
 2 * 29896, calculated from amino acid sequence Mesorhizobium loti
34000
-
 2 * 34000, SDS-PAGE Mesorhizobium loti
64000
-
 native enzyme, gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-
Mesorhizobium loti MAFF303099
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, butyl-Toyopearl column chromatography, hydoxylapatite column chromatography, and Sephadex G25 gel filtration Mesorhizobium loti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.468
-
 crude extract, at 25°C Mesorhizobium loti
1.89
-
 purified enzyme, at 25°C Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(acetamidomethylene)succinate + 2 H2O
-
 Mesorhizobium loti acetate + succinate semialdehyde + NH3 + CO2
-
 ?
2-(acetamidomethylene)succinate + 2 H2O
-
 Mesorhizobium loti MAFF303099 acetate + succinate semialdehyde + NH3 + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Mesorhizobium loti
dimer 2 * 29896, calculated from amino acid sequence Mesorhizobium loti

Synonyms

Synonyms Comment Organism
AAMS amidohydrolase
-
 Mesorhizobium loti
AAMSAH
-
 Mesorhizobium loti
alpha-(N-acetylaminomethylene)succinic acid amidohydrolase
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 in 0.1 M potassium phosphate buffer (pH 7.0) Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme is stable at 50°C for 10 min, the half-lives of the enzyme are 193.6, 77.3, 21.6, and 13.6 min at 50, 55, 58, and 60°C, respectively Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
307.3
-
 alpha-(N-acetylaminomethylene)succinic acid at 25°C Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in 100 mM GTA buffer Mesorhizobium loti
7
-
 in sodium diphosphate buffer Mesorhizobium loti
7.5
-
 in potassium or sodium phosphate buffer Mesorhizobium loti
8
-
 in Tris-HCl Mesorhizobium loti