BRENDA - Enzyme Database
show all sequences of 3.5.1.25

Enzymes of N-acetylglucosamine metabolism during germ-tube formation in Candida albicans

Gopal, P.; Sullivan, P.A.; Shepherd, M.G.; J. Gen. Microbiol. 128, 2319-2326 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
no inhibition
Candida albicans
fructose-6-phosphate
40 mM, 75% inhibition
Candida albicans
glucosamine 6-phosphate
competitive
Candida albicans
UDP-N-acetylglucosamine
10 mM, 35% inhibition
Candida albicans
UTP
7.5 mM, 21% inhibition
Candida albicans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-glucosamine 6-phosphate + H2O
Candida albicans
enzyme of the N-acetylglucosamine pathway
D-glucosamine 6-phosphate + acetate
-
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
Candida albicans
inducible enzyme, trichodermin and lomofungin inhibit induction of the enzyme
D-glucosamine 6-phosphate + acetate
-
Candida albicans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida albicans
-
-
-
Storage Stability
Storage Stability
Organism
4°C, 100 mM Tris/HCl, pH 7.2, stable for 1 week
Candida albicans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-glucosamine 6-phosphate + H2O
-
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
enzyme of the N-acetylglucosamine pathway
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
inducible enzyme, trichodermin and lomofungin inhibit induction of the enzyme
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Candida albicans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
more than 90% of maximal activity below pH 9.5 and above pH 7.0
Candida albicans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
no inhibition
Candida albicans
fructose-6-phosphate
40 mM, 75% inhibition
Candida albicans
glucosamine 6-phosphate
competitive
Candida albicans
UDP-N-acetylglucosamine
10 mM, 35% inhibition
Candida albicans
UTP
7.5 mM, 21% inhibition
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetyl-D-glucosamine 6-phosphate + H2O
Candida albicans
enzyme of the N-acetylglucosamine pathway
D-glucosamine 6-phosphate + acetate
-
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
Candida albicans
inducible enzyme, trichodermin and lomofungin inhibit induction of the enzyme
D-glucosamine 6-phosphate + acetate
-
Candida albicans
?
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 100 mM Tris/HCl, pH 7.2, stable for 1 week
Candida albicans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-glucosamine 6-phosphate + H2O
-
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
enzyme of the N-acetylglucosamine pathway
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
N-acetyl-D-glucosamine 6-phosphate + H2O
inducible enzyme, trichodermin and lomofungin inhibit induction of the enzyme
246591
Candida albicans
D-glucosamine 6-phosphate + acetate
-
246591
Candida albicans
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Candida albicans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
more than 90% of maximal activity below pH 9.5 and above pH 7.0
Candida albicans
Other publictions for EC 3.5.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734468
Choi
Characterization of Streptococ ...
Streptococcus pneumoniae, Streptococcus pneumoniae BAA-255
J. Microbiol.
51
659-664
2013
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1
1
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1
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2
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-
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718709
Popowska
N-acetylglucosamine-6-phosphat ...
Listeria monocytogenes
Arch. Microbiol.
194
255-268
2012
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-
1
-
1
-
-
-
2
-
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2
-
5
-
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2
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2
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2
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3
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2
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2
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-
-
-
-
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-
4
7
-
-
-
720819
Yadav
N-acetylglucosamine 6-phosphat ...
Komagataeibacter xylinus 10245, Komagataeibacter xylinus
PLoS ONE
6
e18099
2011
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-
1
-
-
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-
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6
-
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2
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
710931
Wendland
N-Acetylglucosamine utilizatio ...
Candida albicans
Appl. Environ. Microbiol.
75
5840-5845
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
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-
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-
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1
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1
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-
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1
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-
-
-
-
-
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-
1
1
-
-
-
712288
Plumbridge
An alternative route for recyc ...
Escherichia coli
J. Bacteriol.
191
5641-5647
2009
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-
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1
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1
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1
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-
2
-
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-
-
-
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1
1
-
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-
694019
May
Genetic variation in sialidase ...
Mycoplasma synoviae
Microb. Pathog.
45
38-44
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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678301
Hall
N-Acetyl-D-glucosamine-6-phosp ...
Escherichia coli
Biochemistry
46
7942-7952
2007
-
-
1
-
-
-
-
23
-
1
1
-
-
2
-
-
1
-
-
-
-
-
6
1
-
-
-
27
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
23
-
1
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
27
-
-
-
-
-
-
-
-
-
-
678302
Hall
Structural diversity within th ...
Escherichia coli, Thermotoga maritima
Biochemistry
46
7953-7962
2007
-
-
2
2
-
-
1
1
-
2
-
2
-
4
-
-
2
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
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2
-
2
-
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-
1
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1
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2
-
2
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2
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-
2
1
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1
-
-
-
-
-
-
-
-
-
-
669051
Xu
Tight binding inhibitors of N- ...
Escherichia coli
J. Am. Chem. Soc.
128
4244-4245
2006
-
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1
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1
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1
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1
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1
2
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1
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1
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-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669882
Ferreira
Structural analysis of N-acety ...
Escherichia coli
J. Mol. Biol.
359
308-321
2006
-
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1
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3
-
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2
-
1
-
4
-
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1
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2
1
1
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1
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1
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3
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2
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1
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2
1
1
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1
-
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669096
Alvarez-Anorve
Why does Escherichia coli grow ...
Escherichia coli
J. Bacteriol.
187
2974-2982
2005
-
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1
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1
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1
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652547
Vincent
The three-dimensional structur ...
Bacillus subtilis
J. Biol. Chem.
279
2809-2816
2004
-
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1
1
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2
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1
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1
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246596
Ferreira
-
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
56
670-672
2000
-
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1
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1
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1
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1
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246600
Yamano
Purification and characterizat ...
Alteromonas sp., Vibrio cholerae
Mar. Biotechnol.
2
57-64
2000
-
-
-
-
-
-
6
9
-
2
2
-
-
6
-
-
1
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1
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6
-
2
2
2
9
-
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6
-
9
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2
2
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1
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1
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6
-
2
2
2
9
-
-
-
-
-
-
-
-
-
-
246597
Souza
N-Acetylglucosamine-6-phosphat ...
Escherichia coli, Escherichia coli overproducing
Arch. Biochem. Biophys.
340
338-346
1997
-
-
-
-
-
-
3
3
-
-
2
-
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11
-
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1
1
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4
1
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1
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3
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1
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4
1
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1
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246595
Yamano
Purification and characterizat ...
Vibrio cholerae, Vibrio cholerae Non-O1
Biosci. Biotechnol. Biochem.
60
1320-1323
1996
-
-
-
-
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4
3
-
1
2
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5
-
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1
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1
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6
1
1
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3
3
1
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1
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4
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3
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1
2
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1
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1
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6
1
1
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3
3
1
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1
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-
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-
246598
Weidanz
N-Acetylglucosamine kinase and ...
Homo sapiens, Plasmodium falciparum
Br. J. Haematol.
95
645-653
1996
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-
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5
1
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1
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2
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5
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1
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3
1
1
4
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1
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5
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1
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1
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2
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1
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3
1
1
4
-
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1
-
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-
-
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-
-
246599
Campbell
N-Acetylglucosamine-6-phosphat ...
Homo sapiens, Rattus norvegicus
Hepatology
11
199-204
1990
-
-
-
-
-
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-
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2
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4
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14
-
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3
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2
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14
-
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3
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246589
Campbell
Assay and properties of N-acet ...
Rattus norvegicus
Anal. Biochem.
166
134-141
1987
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6
1
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1
1
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2
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1
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2
1
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1
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6
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1
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1
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1
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1
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246590
Corner
A Candida albicans mutant impa ...
Candida albicans
J. Gen. Microbiol.
132
15-19
1986
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2
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246591
Gopal
Enzymes of N-acetylglucosamine ...
Candida albicans
J. Gen. Microbiol.
128
2319-2326
1982
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5
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2
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3
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1
3
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1
1
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5
-
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2
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1
3
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1
1
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246593
Gopal
A rapid and sensitive assay fo ...
Candida albicans
Anal. Biochem.
115
30-33
1981
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1
1
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1
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1
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1
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1
1
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1
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1
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246594
White
N-Acetylglucosamine-6-phosphat ...
Bacillus subtilis, Bifidobacterium bifidum, Bos taurus, Escherichia coli
Methods Enzymol.
41
497-502
1975
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2
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5
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1
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1
1
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4
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1
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2
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1
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1
1
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4
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1
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246592
White
The purification and propertie ...
Escherichia coli
Biochem. J.
105
121-125
1967
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1
3
1
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2
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1
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1
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1
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1
1
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1
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3
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1
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1
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1
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1
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1
1
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