Cloned (Comment) | Organism |
---|---|
gene tod1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic tree | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of T-DNA insertion mutants harboring the mutation in exn tod1-1 or tod1-2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Arabidopsis thaliana | 5794 | - |
microsome | - |
Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q6DBD7 | gene tod1 | - |
Arabidopsis thaliana Landsberg erecta | Q6DBD7 | gene tod1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
guard cell | from siliques | Arabidopsis thaliana | - |
additional information | enzyme expression analysis | Arabidopsis thaliana | - |
pollen | tubes | Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C12-NBD-Cer + H2O | - |
Arabidopsis thaliana | NBD-dodecanoic acid + sphingosine | - |
? | |
C12-NBD-Cer + H2O | - |
Arabidopsis thaliana Landsberg erecta | NBD-dodecanoic acid + sphingosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme has a transmembrane domain (residues 20-39), and a DUF616 domain (residues 107-409) | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
alkaline ceramidase | - |
Arabidopsis thaliana |
TOD1 | - |
Arabidopsis thaliana |
turgor regulation defect 1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme-deficient tod1 mutant pollen tubes have higher turgor than wild-type and show growth retardation both in pistils and in agarose medium. In addition, tod1 mutant guard cells are insensitive to abscisic acid-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of abscisic acid signalling and product of alkaline ceramidases, promotes closure in both wild-type and tod1, TOD1 is sufficient to rescue pollen tube growth defect. Pollen tube growth defects of tod1-2 are suppressed by the gaut13 mutation, a single-nucleotide substitution in the galacturonosyltransferase13 gene. Phenotypes, overview | Arabidopsis thaliana |
physiological function | alkaline ceramidase TOD1 is preferentially expressed in pollen tubes and in silique guard cells, where it is required for turgor pressure regulation, it is a key turgor pressure regulator in plant cells, molecular mechanism. Turgor pressure plays pivotal roles in the growth and movement of walled cells | Arabidopsis thaliana |