Cloned (Comment) | Organism |
---|---|
gene YPC, recombinant expression of HA7-tagged enzyme | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
G124C | site-directed mutagenesis | Saccharomyces cerevisiae |
G235C | site-directed mutagenesis | Saccharomyces cerevisiae |
K157C | site-directed mutagenesis | Saccharomyces cerevisiae |
K67C | site-directed mutagenesis | Saccharomyces cerevisiae |
K91C | site-directed mutagenesis | Saccharomyces cerevisiae |
L65C | site-directed mutagenesis | Saccharomyces cerevisiae |
additional information | comparison of membrane topology of wild-type and mutant enzymes, overview | Saccharomyces cerevisiae |
N123C | site-directed mutagenesis | Saccharomyces cerevisiae |
N158C | site-directed mutagenesis | Saccharomyces cerevisiae |
R93C | site-directed mutagenesis | Saccharomyces cerevisiae |
S197C | site-directed mutagenesis | Saccharomyces cerevisiae |
S293C | site-directed mutagenesis | Saccharomyces cerevisiae |
S297C | site-directed mutagenesis | Saccharomyces cerevisiae |
T181C | site-directed mutagenesis | Saccharomyces cerevisiae |
T98C | site-directed mutagenesis | Saccharomyces cerevisiae |
V185C | site-directed mutagenesis | Saccharomyces cerevisiae |
V236C | site-directed mutagenesis | Saccharomyces cerevisiae |
Y182C | site-directed mutagenesis | Saccharomyces cerevisiae |
Y184C | site-directed mutagenesis | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the reverse ceramidase activity in microsomes can only be blocked by a lysine residuespecific reagent when the reagent has access to Ypc1p from the lumenal side | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
endoplasmic reticulum | the N- and C-terminal ends of Ypc1p are oriented towards the lumen and cytosol, respectively, cytosolic or lumenal loops | Saccharomyces cerevisiae | 5783 | - |
membrane | enzyme membrane topology, overview | Saccharomyces cerevisiae | 16020 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acylsphingosine + H2O | Saccharomyces cerevisiae | the carboxylate is a fatty acid | carboxylate + sphingosine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38298 | gene YPC1 | - |
Saccharomyces cerevisiae | Q02896 | gene YDC1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acylsphingosine + H2O | the carboxylate is a fatty acid | Saccharomyces cerevisiae | carboxylate + sphingosine | - |
r | |
N-acylsphingosine + H2O | the carboxylate is a fatty acid, Ypc1p can catalyse the reverse reaction, i.e. the condensation of non-esterified fatty acids with phytosphingosine or dihydrosphingosine. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity | Saccharomyces cerevisiae | carboxylate + sphingosine | - |
r | |
N-acylsphingosine + H2O | the carboxylate is a fatty acid. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity | Saccharomyces cerevisiae | carboxylate + sphingosine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
alkaline ceramidase | - |
Saccharomyces cerevisiae |
Ydc1p | - |
Saccharomyces cerevisiae |
yeast dihydroceramidase 1 | - |
Saccharomyces cerevisiae |
yeast phyto-ceramidase 1 | - |
Saccharomyces cerevisiae |
Ypc1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CREST superfamily | Saccharomyces cerevisiae |
malfunction | overexpression of Ydc1p protein significantly increases the levels of free long-chain bases and long-chain base-phosphates and reduces the biosynthetic flow of long-chain bases towards mature sphingolipids | Saccharomyces cerevisiae |
malfunction | overexpression of Ypc1p protein significantly increases the levels of free long-chain bases and long-chain base-phosphates and reduces the biosynthetic flow of long-chain bases towards mature sphingolipids, whereas deletion of gene YPC1 causes a significant increase in mature sphingolipids | Saccharomyces cerevisiae |
additional information | two of the five natural cysteines, Cys27 and Cys219, are essential for enzymatic activity and form a disulfide bridge. Enzyme Ypc1p possesses the Pfam PF05875 ceramidase motif containing seven predicted transmembranes | Saccharomyces cerevisiae |