Literature summary for 3.5.1.23 extracted from

Tada, S.; Matsushita-Morita, M.; Suzuki, S.; Kusumoto, K.; Kashiwagi, Y.
Characterization of a neutral ceramidase orthologue from Aspergillus oryzae (2009), FEMS Microbiol. Lett., 298, 157-165.

Search for more literature for 3.5.1.23

Activating Compound

Activating Compound	Comment	Organism	Structure
cardiolipin	-	Aspergillus oryzae RIB40
additional information	phosphatidylethanolamine and phosphatidylcholine have little effect on the activity regardless of the concentration	Aspergillus oryzae RIB40
phosphatidylglycerol	-	Aspergillus oryzae RIB40
phosphatidylserine	-	Aspergillus oryzae RIB40

Cloned(Commentary)

Cloned (Comment)	Organism
gene asahA, DNA and amino acid sequence determination and analysis, overexpression of HA- and His6-tagged neutral CDase in Aspergillus oryzae	Aspergillus oryzae RIB40

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	15% inhibition at 5 mM	Aspergillus oryzae RIB40
Hg2+	50% inhibition at 5 mM	Aspergillus oryzae RIB40
Mg2+	10% inhibition at 5 mM	Aspergillus oryzae RIB40
Mn2+	25-30% inhibition at 5 mM	Aspergillus oryzae RIB40
phosphate	80% inhibition at 100 mM	Aspergillus oryzae RIB40

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Aspergillus oryzae RIB40
0.00332	-	N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine	pH 4.0, 40°C	Aspergillus oryzae RIB40

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	activate about 18% at 5 mM	Aspergillus oryzae RIB40
additional information	Zn2+, and EDTA have little effect on the activity	Aspergillus oryzae RIB40

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
97000	-	x * 97000, recombinant enzyme, SDS-PAGE	Aspergillus oryzae RIB40

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae RIB40	Q2UN98	gene asahA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HA- and His6-tagged AsahA 90fold from Aspergillus oryzae	Aspergillus oryzae RIB40

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
C24-ceramide + H2O	-	Aspergillus oryzae RIB40	C24 fatty acid + sphingosine	-	r
hexadecanoyl-ceramide + H2O	i.e. C16-ceramide	Aspergillus oryzae RIB40	hexadecanoate + sphingosine	-	r
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine + H2O	i.e. C12-NBD-ceramide	Aspergillus oryzae RIB40	N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + sphingosine	-	r
octadecanoyl-ceramide + H2O	i.e. C18-ceramide	Aspergillus oryzae RIB40	octadecanoate + sphingosine	-	r

Subunits

Subunits	Comment	Organism
?	x * 97000, recombinant enzyme, SDS-PAGE	Aspergillus oryzae RIB40

Synonyms

Synonyms	Comment	Organism
AsahA	-	Aspergillus oryzae RIB40
CDase	-	Aspergillus oryzae RIB40
neutral CDase	-	Aspergillus oryzae RIB40
neutral ceramidase	-	Aspergillus oryzae RIB40

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	hydrolysis and synthesis reactions	Aspergillus oryzae RIB40

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	hydrolysis reaction	Aspergillus oryzae RIB40
5	-	synthesis reaction, with NBD-dodecanoic acid and sphingosine as substrates	Aspergillus oryzae RIB40

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	7	activity range of neutral CDase, profile overview	Aspergillus oryzae RIB40

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Release 2023.1 (January 2023)