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Literature summary for 3.5.1.136 extracted from

  • Mine, S.; Ikegami, T.; Kawasaki, K.; Nakamura, T.; Uegaki, K.
    Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii (2012), Protein Expr. Purif., 84, 265-269 .
    View publication on PubMed
Search for more literature for 3.5.1.136

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys and successfully refolded in vitro Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
174000
-
 gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii ATCC 700860
-
 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58235
-
-
Pyrococcus horikoshii ATCC 700860 O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
 ?

Subunits

Subunits Comment Organism
homohexamer
-
 Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
diacetylchitobiose deacetylase
-
 Pyrococcus horikoshii
PH0499
-
 Pyrococcus horikoshii