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Literature summary for 3.5.1.134 extracted from

  • Chou, J.; Kuleck, G.; Cohen, J.; Mulbry, W.
    Partial purification and characterization of an inducible indole-3-acetyl-L-aspartic acid hydrolase from Enterobacter agglomerans (1996), Plant Physiol., 112, 1281-1287 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.134

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 60% inhibition in presence of Mg2+ Pantoea agglomerans
dithiothreitol 1 mM, 80% inhibition in presence of Mg2+ and 50% inhibition in absence of divalent cations Pantoea agglomerans
Triton X-100 1%, 50% inhibition in absence of divalent cations Pantoea agglomerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.5
-
 (indol-3-yl)acetyl-L-aspartate pH 7.0, 30°C Pantoea agglomerans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 40% increase in activity Pantoea agglomerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000 55000 gel filtration Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans O50173
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)acetyl-L-aspartate + H2O
-
 Pantoea agglomerans (indol-3-yl)acetate + L-aspartate
-
 ?
additional information enzyme is highly specific for (indol-3-yl)acetyl-L-aspartate. No substrates: indole-3-acetic acid, ammonium sulfate, urea, indoleacetamide Pantoea agglomerans ?
-
 ?
N-acetyl-L-aspartate + H2O
-
 Pantoea agglomerans acetate + L-aspartate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Pantoea agglomerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Pantoea agglomerans

Expression

Organism Comment Expression
Pantoea agglomerans expression is induced by growth on (indol-3-yl)acetyl-L-aspartate or N-acetyl-L-aspartate up