Literature summary for 3.5.1.130 extracted from

Fujita, S.; Cho, S.H.; Yoshida, A.; Hasebe, F.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
Crystal structure of LysK, an enzyme catalyzing the last step of lysine biosynthesis in Thermus thermophilus, in complex with lysine Insight into the mechanism for recognition of the amino-group carrier protein, LysW (2017), Biochem. Biophys. Res. Commun., 491, 409-415 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
LysK in complex with lysine at a resolution of 2.4 A. The alpha-amino group of the bound lysine is oriented toward the catalytic center, which is composed of the residues coordinating divalent metal ions for the hydrolysis of the isopeptide bond. An 11 A long path leads from the active site binding lysine to the protein surface. A positively-charged surface region is located around the exit of the path	Thermus thermophilus

Crystallization (Comment)

Organism

LysK in complex with lysine at a resolution of 2.4 A. The alpha-amino group of the bound lysine is oriented toward the catalytic center, which is composed of the residues coordinating divalent metal ions for the hydrolysis of the isopeptide bond. An 11 A long path leads from the active site binding lysine to the protein surface. A positively-charged surface region is located around the exit of the path

Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
D325A	marked decrease in activity	Thermus thermophilus
K297E	marked decrease in activity	Thermus thermophilus
R282E	marked decrease in activity	Thermus thermophilus
R286E	marked decrease in activity	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q8VUS5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O	-	Thermus thermophilus	[carrier protein LysW]-C-terminal-L-glutamate + L-lysine	-	?
[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O	i.e. the C-terminal 10 residues of LysW fused with one lysine residue at its gamma-carboxyl group of C-terminal Glu54	Thermus thermophilus	[CT10-LysW]-C-terminal-L-glutamate + L-lysine	-	?

Synonyms

Synonyms	Comment	Organism
lysK	-	Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
3.1	-	[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	mutant R286E, pH 8.0, 60°C	Thermus thermophilus
4.5	-	[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	mutant R282E, pH 8.0, 60°C	Thermus thermophilus
5.5	-	[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	wild-type, pH 8.0, 60°C	Thermus thermophilus
36	-	[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	mutant R286E, pH 8.0, 60°C	Thermus thermophilus
38	-	[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	mutant R282E, pH 8.0, 60°C	Thermus thermophilus
530	-	[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate	wild-type, pH 8.0, 60°C	Thermus thermophilus