Literature summary for 3.5.1.128 extracted from

Niehaus, T.D.; Patterson, J.A.; Alexander, D.C.; Folz, J.S.; Pyc, M.; MacTavish, B.S.; Bruner, S.D.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
The metabolite repair enzyme Nit1 is a dual-targeted amidase that disposes of damaged glutathione in Arabidopsis (2019), Biochem. J., 476, 683-697 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 22°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	both cytoplasmic and plastidial localization. The N-(4-oxoglutaryl)-L-cysteinylglycine repair system is directed to at least two cellular compartments (cytoplasm, plastid) via the use of alternative translation start sites	Arabidopsis thaliana	5737	-
plastid	both cytoplasmic and plastidial localization. The N-(4-oxoglutaryl)-L-cysteinylglycine repair system is directed to at least two cellular compartments (cytoplasm, plastid) via the use of alternative translation start sites	Arabidopsis thaliana	9536	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q94JV5	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	the recombinant enzyme has high and specific amidase activity towards N-(4-oxoglutaryl)-L-cysteinylglycine	Arabidopsis thaliana	2-oxoglutarate + L-cysteinylglycine	-	?

Synonyms

Synonyms	Comment	Organism
At4g08790	-	Arabidopsis thaliana
NIT1	-	Arabidopsis thaliana
nitrilase-like protein 1	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 22°C	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Arabidopsis thaliana

General Information

General Information	Comment	Organism
physiological function	ablating the Arabidopsis Nit1 gene causes a massive accumulation of N-(4-oxoglutaryl)-L-cysteinylglycine and other marked changes to the metabolome	Arabidopsis thaliana
physiological function	the N-(4-oxoglutaryl)-L-cysteinylglycine repair system is directed to at least two cellular compartments (cytoplasm, plastid) via the use of alternative translation start sites	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
121	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 22°C	Arabidopsis thaliana

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Release 2023.1 (January 2023)