Crystallization (Comment) | Organism |
---|---|
wild-type and mutant S121P. Residue 121 impacts the conformation of the conserved tyrosine 27, a component of the catalytically-important flexible N-terminal loop | Wolinella succinogenes |
Protein Variants | Comment | Organism |
---|---|---|
S121P | mutant gains L-glutaminase activity, but retains L-asparaginase activity comparable to wild-type | Wolinella succinogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0217 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
0.0236 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
0.32 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
0.38 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wolinella succinogenes | P50286 | - |
- |
Wolinella succinogenes DSM 1740 | P50286 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Wolinella succinogenes | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Wolinella succinogenes DSM 1740 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | reaction of EC 3.5.1.2 | Wolinella succinogenes | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | reaction of EC 3.5.1.2 | Wolinella succinogenes DSM 1740 | L-glutamate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.58 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
11.33 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
86.7 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
97.8 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
L-glutamine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes | |
35.3 | - |
L-glutamine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
3700 | - |
L-asparagine | mutant S121P, 37°C, pH not specified in the publication | Wolinella succinogenes | |
4500 | - |
L-asparagine | wild-type, 37°C, pH not specified in the publication | Wolinella succinogenes |