Application | Comment | Organism |
---|---|---|
medicine | the purified enzyme shows cytotoxicity for the MOLT-4 leukemic cell lineage | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
K215A | 99.9% loss of activity | Saccharomyces cerevisiae |
additional information | residues T64, Y78, T141, K15 are involved in catalysis | Saccharomyces cerevisiae |
T141A | 99.9% loss of activity | Saccharomyces cerevisiae |
T64A | 99.9% loss of activity | Saccharomyces cerevisiae |
Y78A | 99.9% loss of activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
L-asparagine | K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae | |
0.122 | - |
L-asparagine | presence of L-glutamine, K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
196.2 | - |
pH 8.8, 37°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Saccharomyces cerevisiae | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Saccharomyces cerevisiae BY4741 | L-aspartate + NH3 | - |
? | |
additional information | very poor substrate: L-glutamine | Saccharomyces cerevisiae | ? | - |
? | |
additional information | very poor substrate: L-glutamine | Saccharomyces cerevisiae BY4741 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41400, calculated from sequence, x * 45000, His-tagged recombinant protein, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ASNase1 | - |
Saccharomyces cerevisiae |
ASP1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
217 | - |
L-asparagine | K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae | |
523 | - |
L-asparagine | presence of L-glutamine, K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
L-asparagine | presence of L-glutamine, K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae | |
16 | - |
L-asparagine | K0.5 value, pH 8.8, 37°C | Saccharomyces cerevisiae |