Application | Comment | Organism |
---|---|---|
medicine | the enzyme is used as therapeutic agent in the treatment of acute childhood lymphoblastic leukemia | Pectobacterium carotovorum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pectobacterium carotovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic analysis, kinetic study and molecular modelling | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Pectobacterium carotovorum | - |
L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Pectobacterium carotovorum NCYC 1526 | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Pectobacterium carotovorum NCYC 1526 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity by cation exchange and L-asparagine affinity chromatography | Pectobacterium carotovorum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-asparagine + H2O = L-aspartate + NH3 | kinetic model, substrate specificity, and catalytic mechanism, active site residues are Asp115 and Glu82, the structural transition of the enzyme is rate-limiting in the reaction, entropy changes are the main determinants for the substrate specificity | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Pectobacterium carotovorum NCYC 1526 | L-aspartate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ErA | - |
Pectobacterium carotovorum |
L-asparaginase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pectobacterium carotovorum |