Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenibacillus sp. B38 |
Protein Variants | Comment | Organism |
---|---|---|
H276A | about 35% of wild-type specific activity | Paenibacillus sp. B38 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoyl-Arg-His-[D-Asp]-CH2Cl | 0.1 mM, 48% residual activity | Paenibacillus sp. B38 | |
Mn2+ | 0.1 mM, 68% residual activity | Paenibacillus sp. B38 | |
additional information | not inhibitory: pepstatin, Co2+ | Paenibacillus sp. B38 | |
Zn2+ | 0.1 mM, 46% residual activity | Paenibacillus sp. B38 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | mutant H276A, pH 7.0, 37°C | Paenibacillus sp. B38 | |
0.97 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.0, 37°C | Paenibacillus sp. B38 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Paenibacillus sp. B38 | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. B38 | A0A2Z6BCG6 | - |
- |
Purification (Comment) | Organism |
---|---|
purification from cell filtrate | Paenibacillus sp. B38 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
737 | - |
mutant H276A, pH 8.0, 37°C | Paenibacillus sp. B38 |
2225 | - |
wild-type, pH 8.0, 37°C | Paenibacillus sp. B38 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme specifically recognizes an internal D-Asp residue to cleave [D-Asp]-X peptide bonds | Paenibacillus sp. B38 | ? | - |
? | |
succinyl-D-Asp-7-amido-4-methylcoumarin + H2O | - |
Paenibacillus sp. B38 | succinyl-D-Asp + 7-amino-4-methylcoumarin | - |
? | |
succinyl-D-Asp-p-nitroanilide + H2O | - |
Paenibacillus sp. B38 | succinyl-D-Asp + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, mature recombinant protein, x * 55000, recombinant pro-protein, SDS-PAGE, x * 55756, pro-orotein, calculated from sequence | Paenibacillus sp. B38 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable below | Paenibacillus sp. B38 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | mutant H276A, pH 7.0, 37°C | Paenibacillus sp. B38 | |
2.88 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.0, 37°C | Paenibacillus sp. B38 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paenibacillus sp. B38 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.37 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | mutant H276A, pH 7.0, 37°C | Paenibacillus sp. B38 | |
2.97 | - |
succinyl-D-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.0, 37°C | Paenibacillus sp. B38 |