Cloned (Comment) | Organism |
---|---|
real-time PCR MMP-20 expression analysis, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | MMP-20 is synthesized and secreted by ameloblasts | Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amelogenin + H2O | Mus musculus | MMP-20 is involved in amelogenin processing and degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
newborn C57/Bl6J mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ameloblast | - |
Mus musculus | - |
enamel layer | - |
Mus musculus | - |
odontoblast | - |
Mus musculus | - |
tooth | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amelogenin + H2O | - |
Mus musculus | ? | - |
? | |
amelogenin + H2O | MMP-20 is involved in amelogenin processing and degradation | Mus musculus | ? | - |
? | |
casein + H2O | zymography | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMP-20 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | loss and siRNA-mediated silencing of Runx2 in ameloblast-lineage cells decreases ODAM expression, resulting in downregulation of MMP-20 expression | down |
Mus musculus | increased expression of ODAM and Runx2 augments MMP-20 expression, and both TGF-beta1 and BMP-2 up-regulate Runx2 expression. TGF-beta1 promotes MMP-20 expression in ameloblasts | up |
General Information | Comment | Organism |
---|---|---|
physiological function | MMP-20 is involved in the enamel formation and mineralization. The amelogenin degradation induced by MMP-20 is believed to be essential for the axial growth of enamel crystals MMP-20 activity is regulated by RUNX2, which is stimulated by BMP-2 or TGF-beta, and the odontogenic ameloblast-associated protein, ODAM, molecular mechanisms responsible for MMP-20 regulation, overview. Runx2 regulates ODAM expression, which in turn regulates MMP-20 expression and is recruited to the MMP-20 promoter. Increased MMP-20 expression accelerates amelogenin processing during enamel mineralization | Mus musculus |