Any feedback?
Literature summary for 3.4.24.B3 extracted from
- Production of a bioactive recombinant chicken matrix metalloproteinase-11 peptide in Escherichia coli (2017), Biotechnol. Appl. Biochem., 64, 555-563 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | being selectively expressed in tumor tissues, MMP-11 is a promising target for immunotherapy against tumors | Gallus gallus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| from chicken embryo, functional recombinant overexpression of His-tagged enzyme lacking the propeptide and the signal pepitde in Escherichia coli strain BL21(DE3) T7 Express lysY | Gallus gallus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant production of a thioredoxin-tagged bioactive recombinant chicken MMP-11 (cMMP-11) peptide excluding the secretory signal and propeptide | Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme has a secretory signal | Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Gallus gallus |  |
| Zn2+ | a zinc-dependent endopeptidase | Gallus gallus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | F1NBH2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the proform of the enzyme has a propeptide | Gallus gallus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged modified enzyme from Escherichia coli BL21(DE3) T7 Express lysY by nickel affinity chromatography under denaturing condition (8 M urea, 100 mM phosphate buffer, 10 mM Tris-HCl, pH 7.8) and slow dialysis | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Gallus gallus | ? | - |
? | |
| Gelatin + H2O | - |
Gallus gallus | ? | - |
? | |
| additional information | the recombinant peptide exhibits both caseinolytic and gelatinase activities without requiring activation by 4-aminophenylmercuric acetate | Gallus gallus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54500, recombinant His-tagged enzyme lacking the propeptide and the signal pepitde, SDS-PAGE | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MMP-11 | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Gallus gallus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
