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Literature summary for 3.4.24.B3 extracted from

  • Gall, A.L.; Ruff, M.; Moras, D.
    The dual role of CHAPS in the crystallization of stromelysin-3 catalytic domain (2003), Acta Crystallogr. Sect. D, 59, 603-606.
    View publication on PubMed
Search for more literature for 3.4.24.B3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with a phosphinic inhibitor, inhibitor to protein ratio is 2.2:1, in presence of detergent CHAPS, hanging drop method, 5 mg/ml protein in 0.05 M MES, pH 5.5, 0.5 M ammonium sulfate, 1% v/v trifluoroethanol, X-ray diffraction structure determination at 2.6 A resolution and analysis Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
phosphinic inhibitor
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc binding motif H219EXXHXGXXH229 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q02853 precursor
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli, addition of CHAPS, i.e. 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate, to avoid protein aggregation, binding mechanism Mus musculus

Renatured (Commentary)

Renatured (Comment) Organism
renaturation from inclusion bodies after expression in Escherichia coli Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protein + H2O
-
 Mus musculus peptides
-
 ?

Synonyms

Synonyms Comment Organism
M10.007 Merops-ID Mus musculus
matrix metalloproteinase-11
-
 Mus musculus
MMP-11
-
 Mus musculus
ST3
-
 Mus musculus