Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B28 extracted from

  • Moss, M.L.; Miller, M.A.; Vujanovic, N.; Yoneyama, T.; Rasmussen, F.H.
    Fluorescent substrates for ADAM15 useful for assaying and high throughput screening (2016), Anal. Biochem., 514, 42-47 .
    View publication on PubMed
Search for more literature for 3.4.24.B28

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell or CHO cell Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-[[4-(2-butynyloxy)phenyl]sulfonyl]-N-hydroxy-2,2-dimethyl-(3S)-thiomorpholinecarboxamide i.e. TMI-1, complete inhibition at 0.01 mM Homo sapiens
GI254023 40% inhibition at 0.1 mM Homo sapiens
additional information not inhibitory: TIMP1, TIMP3, marimastat, TAPI2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
 dabcyl-Ala-Pro-Arg-Trp-Ile-Gln-Asp-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.019
-
 dabcyl-Gly-Pro-Leu-Gly-Met-Arg-Gly-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.029
-
 Dabcyl-Leu-Arg-Glu-Gln-Gln-Arg-Leu-Lys-Ser-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13444
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification recombinant enzyme is activated both via thermolysin- and auto-activation. There is no detectable ADAM15 activity in the absence of activation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabcyl-Ala-Pro-Arg-Trp-Ile-Gln-Asp-Lys(5FAM)-NH2 + H2O FRET substrate Homo sapiens ?
-
 ?
dabcyl-Gly-Pro-Leu-Gly-Met-Arg-Gly-Lys(5FAM)-NH2 + H2O FRET substrate Homo sapiens ?
-
 ?
Dabcyl-Leu-Arg-Glu-Gln-Gln-Arg-Leu-Lys-Ser-Lys(5FAM)-NH2 + H2O FRET substrate, based on a physiological CD23 cleavage site Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.032
-
 dabcyl-Ala-Pro-Arg-Trp-Ile-Gln-Asp-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.11
-
 dabcyl-Gly-Pro-Leu-Gly-Met-Arg-Gly-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.15
-
 Dabcyl-Leu-Arg-Glu-Gln-Gln-Arg-Leu-Lys-Ser-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4300
-
 dabcyl-Ala-Pro-Arg-Trp-Ile-Gln-Asp-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
5200
-
 Dabcyl-Leu-Arg-Glu-Gln-Gln-Arg-Leu-Lys-Ser-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens
5800
-
 dabcyl-Gly-Pro-Leu-Gly-Met-Arg-Gly-Lys(5FAM)-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens