Literature summary for 3.4.24.B24 extracted from

Fukushima, T.; Yao, Y.; Kitajima, T.; Yamamoto, H.; Sekiguchi, J.
Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis (2007), Mol. Genet. Genomics, 278, 371-383.

Search for more literature for 3.4.24.B24

Cloned(Commentary)

Cloned (Comment)	Organism
truncated CwlK fused with a histidine-tag, h-deltaCwlK, is produced in Escherichia coli cells, fusions with the 3xFLAG-tag are constructed	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bacillus subtilis	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17600	-	h-deltaCwlK, determined by SDS-PAGE and zymography	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34360	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
lipoprotein	-	Bacillus subtilis

Purification (Commentary)

Purification (Comment)	Organism
purified on a nickel column	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the h-deltaCwlK protein hydrolyzes the peptidoglycan of Bacillus subtilis	Bacillus subtilis	?	-	?

Synonyms

Synonyms	Comment	Organism
CwlK	-	Bacillus subtilis
L,D-endopeptidase	-	Bacillus subtilis
L-alanoyl-D-glutamate peptidase	-	Bacillus subtilis
Peptidoglycan hydrolase	-	Bacillus subtilis
peptidoglycan L-alanyl-D-glutamate endopeptidase cwIK	-	Bacillus subtilis
YcdD	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Bacillus subtilis

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Release 2023.1 (January 2023)