Literature summary for 3.4.24.B20 extracted from

Vostrukhina, M.; Popov, A.; Brunstein, E.; Lanz, M.A.; Baumgartner, R.; Bieniossek, C.; Schacherl, M.; Baumann, U.
The structure of Aquifex aeolicus FtsH in the ADP-bound state reveals a C2-symmetric hexamer (2015), Acta Crystallogr. Sect. D, 71, 1307-1318.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using either 2 M ammonium sulfate, 0.1 M Tris-HCl pH 8.5, 10 mM EDTA or, as another condition, 60% tacsimate pH 7.0, 10 mM adenylyl imidodiphosphate lithium salt hydrate	Aquifex aeolicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains zinc	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O67077	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Resource Q anion-exchange chromatography, and Superdex 16/6000 gel filtration	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
casein + H2O	-	Aquifex aeolicus	?	-	?

Subunits

Subunits	Comment	Organism
hexamer	x-ray crystallography	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
FtsH	-	Aquifex aeolicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Aquifex aeolicus

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Release 2023.1 (January 2023)