Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of the staphylolytic activity | Pseudomonas aeruginosa | |
dithiothreitol | inhibition of the staphylolytic activity | Pseudomonas aeruginosa | |
EDTA | inhibition only above 20 mM | Pseudomonas aeruginosa | |
EGTA | inhibition only above 20 mM | Pseudomonas aeruginosa | |
additional information | no inhibition by 1,7-penanthroline and 4,7-phenanthroline, activity with beta-caseinis blocked by inhibitors that do not block the staphylolytic activity, no inhibition by several serine or cysteine proteinase inhibitors including diisopropyl fluorophosphate, phenylmethylsulfonylfluoride, leupeptin, and N-ethylmaleimide | Pseudomonas aeruginosa | |
Nalpha-p-tosyl-L-lysine chloromethyl ketone | inhibition of the staphylolytic activity | Pseudomonas aeruginosa | |
phosphoramidon | inhibition of the staphylolytic activity | Pseudomonas aeruginosa | |
Tetraethylenepentamine | inhibition of the staphylolytic activity | Pseudomonas aeruginosa | |
Zn2+ | in excess, inhibition of staphylolytic activity | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-metalloprotease | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Elastin + H2O | Pseudomonas aeruginosa | enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin | ? | - |
? | |
additional information | Pseudomonas aeruginosa | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides | ? | - |
? | |
protein + H2O | Pseudomonas aeruginosa | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | specific for Gly-Ala peptide bonds within Gly-Gly-Ala sequences in elastin, substrates that contain no Gly-Gly peptide bond, such as beta-casein, appear to be resistant to the enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Elastin + H2O | soluble recombinant and insoluble elastin | Pseudomonas aeruginosa | ? | - |
? | |
Elastin + H2O | enzyme activates elastase, and the elastolytic activity of other proteases by cleaving elastin | Pseudomonas aeruginosa | ? | - |
? | |
additional information | enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells by cleaving the peptidoglycan pentaglycine interpeptides | Pseudomonas aeruginosa | ? | - |
? | |
protein + H2O | - |
Pseudomonas aeruginosa | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M23.002 | Merops-ID | Pseudomonas aeruginosa |
staphylolysin | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pseudomonas aeruginosa |