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Literature summary for 3.4.24.B15 extracted from
- Characterization of PHEX endopeptidase catalytic activity: identification of parathyroid-hormone-related peptide107-139 as a substrate and osteocalcin, PPi and phosphate as inhibitors (2001), Biochem. J., 355, 707-713.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect of Ca2+ alone | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PHEX gene, expression of wild-type and mutants in LLC-PK1 porcine kidney cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E581V | site-directed mutagenesis, soluble secreted mutant secPHEXE581V, inactive | Homo sapiens |
| additional information | construction of an active soluble secreted mutant enzyme form secPHEX, enzyme mutations are responsible for X-chromosome linked hypophosphataemia | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | complete inhibition at 1 mM | Homo sapiens |  |
| diphosphate | 50% inhibition at 2.5 mM | Homo sapiens | |
| EDTA | complete inhibition at 1 mM | Homo sapiens | |
| additional information | no effect of Ca2+ alone | Homo sapiens | |
| osteocalcin | 50% inhibition at 0.0036 mM, Ca2+ reverses the effect | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-metalloprotease | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
| 100000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
| 105000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Homo sapiens | enzyme mutations are responsible for X-chromosome linked hypophosphataemia | peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble secreted mutant enzyme forms | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| proteolytic degradation of proteins | at the N-terminus of aspartate residues, the S1' pocket can accomodate negatively charged side chains of amino acid residues | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with [Leu]enkephalin, alpha-endorphin, substance P, bradykinin, big-endothelin-1, endothelin-1, alpha-calcitonin, gene-related peptide alpha-CGRP, calcitonin, osteocalcin, parathyroid-hormone-related peptide 1-84, parathyroid-hormone-related peptide 1-34, and osteogenic growth peptide | Homo sapiens | ? | - |
? | |
| parathyroid-hormone-related peptide 107-139 + H2O | - |
Homo sapiens | L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucyl-L-alpha-aspartyl-L-serylglycyl-L-valyl-L-threonylglycyl-L-serylglycyl-L-leucyl-L-alpha-glutamylglycyl-L-a-aspartyl-L-histidyl-L-leucyl-L-serine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucyl-L-alpha-aspartyl-L-seryl-L-arginine + L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucine + L-alpha-aspartyl-L-seryl-L-arginine | product determination | ? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
| protein + H2O | enzyme mutations are responsible for X-chromosome linked hypophosphataemia | Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M13.091 | Merops-ID | Homo sapiens |
| PHEX | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | activity rapidly decreases at more basic pH values | Homo sapiens |
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