Cloned (Comment) | Organism |
---|---|
stable expression of His-tagged ADAMTS13 in HEK-293 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
FRETSVWF73 peptide | pH 7.5, 37°C, recombinant ADAMTS13 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
von Willebrand factor + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged ADAMTS13 from HEK-293 cells by anion exchange chromatography, Ni affinity chromatography, and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FRETSVWF73 peptide + H2O | a von Willebrand factor-derived fluorescein-labeled peptide substrate | Homo sapiens | ? | - |
? | |
von Willebrand factor + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS13 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.43 | - |
FRETSVWF73 peptide | pH 7.5, 37°C, recombinant ADAMTS13 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | under denaturing and fluid shear stress conditions multiple leukocyte proteases cleave von Willebrand factor predominantly in the central A2 domain. Activated neutrophils, but not normal neutrophils, retain von Willebrand factor-cleaving activity in the presence of plasma inhibitors, suggesting that leukocyte proteases may regulate von Willebrand factor function under physiologic conditions, kinetics and reaction products, overview | Homo sapiens |
physiological function | importance of ADAMTS13 metalloprotease in von Willebrand factor regulation and association between severe deficiency of ADAMTS13 and thrombotic thrombocytopenic purpura. However, ADAMTS13 activity levels do not always correlate with the clinical course of TTP, suggesting that other proteases, e.g. elastase or proteinase 3 expressed on leukocyte surfaces, might be important in regulating von Willebrand factor | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
763.8 | - |
FRETSVWF73 peptide | pH 7.5, 37°C, recombinant ADAMTS13 | Homo sapiens |