Cloned (Comment) | Organism |
---|---|
gene MMP14, recombinant expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-TIMP-2 | - |
Homo sapiens | |
N-TIMP-3 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and thermodynamics over a wide range of temperatures and pressures, stopped-flow fluorescence technique | Homo sapiens | |
0.0006 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 10°C, recombinant enzyme | Homo sapiens | |
0.0018 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
0.0021 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 30°C, recombinant enzyme | Homo sapiens | |
0.0028 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 37°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular matrix | - |
Homo sapiens | 31012 | - |
membrane | a transmembrane endopeptidase | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | a calcium- and zinc-dependent transmembrane endopeptidase | Homo sapiens | |
Zn2+ | a calcium- and zinc-dependent transmembrane endopeptidase | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50281 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 + H2O | - |
Homo sapiens | methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly + Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | - |
? | |
additional information | modeling of enzyme-substrate interaction | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane type 1-matrix metalloproteinase | - |
Homo sapiens |
MMP-14 | - |
Homo sapiens |
MT1-MMP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | activity range | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 10°C, recombinant enzyme | Homo sapiens | |
2.1 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
3.4 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 30°C, recombinant enzyme | Homo sapiens | |
6.8 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 37°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | a small conformational change is detected at 37°C, which is responsible for the change in activity observed at the same temperature. Pressure decreases the enzymatic activity until complete inactivation occurs at 2 kbar. The inactivation is associated with changes in the rate-limiting step of the reaction caused by additional hydration of the active site upon compression and/or minor conformational changes in the active site region | Homo sapiens |
physiological function | membrane type 1-matrix metalloproteinase (MT1-MMP or MMP-14) is involved in the degradation of extracellular matrix and tumor invasion. MT1-MMP is involved in the mediation of pericellular proteolysis of extracellular matrix components, essential for the physiological remodeling processes such as tissue repair, development, and morphogenesis. MT1-MMP is overexpressed in many cancer types and increases tumor cell growth, invasion, and metastasis by degrading extracellular matrix components and making paths through surrounding tissues | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
550 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 10°C, recombinant enzyme | Homo sapiens | |
1167 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
1619 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 30°C, recombinant enzyme | Homo sapiens | |
2429 | - |
methoxycoumarin-4-acetyl-Lys-Pro-Leu-Gly-Leu-Lys(2,4-dinitrophenyl)-Ala-Arg-NH2 | pH 7.0, 37°C, recombinant enzyme | Homo sapiens |