Activating Compound | Comment | Organism | Structure |
---|---|---|---|
TAPI-1 | leads to higher amounts of the MT1-MMP 32 kDa form in the media, also slightly increases the amount of the 50 kDa species of MT1-MMP | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in non-malignant monkey kidney epithelial BS-C-1 and CV-1 cells by co-infecting the cells with the vTF7-3 and vTF-MT1 vaccinia viruses | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | specific amino acid substitutions at the Val524-Ile525 site have no effect on shedding of the 50 kDa species, whereas deletion of the entire stem region deltastem-MT1, which lacks Pro509-Gly535, completely abrogates shedding of the 50 kDa species | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BB94 | - |
Homo sapiens | |
TAPI-1 | inhibits the shedding of the MT1-MMP 18 kDa form | Homo sapiens | |
TIMP-2 | specificity of inhibitor binding of MT1-MMP, shedding of MT1-MMP ectodomain alters the balance of TIMP-2 between the cell surface and the pericellular space | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
immunoblot analysis of untreated cells, soluble MT1-MMP species, product of autocatalytic processing | Homo sapiens |
27000 | - |
immunoblot analysis of cells regardless of TAPI-1 treatment, contains haemopexin-like domain, formed by autocatalytic processing of the 57 kDa active form | Homo sapiens |
32000 | - |
immunoblot analysis of untreated cells | Homo sapiens |
50000 | - |
immunoblot analysis of untreated cells, shed ectodomain | Homo sapiens |
53000 | - |
immunoblot analysis, species of MT1-MMP isolated from a non-detergent extract of human breast carcinoma tissue, lack of the cytosolic tail | Homo sapiens |
57000 | - |
immunoblot analysis of cells treated with TAPI-1, membrane anchored fragment shed in membrane vesicles | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inhibitor-tethered affinity purification with BB94 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast carcinoma cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galectin-3 | cleaved to the 22 kDa degradation product when exposed to cells expressing membrane-anhored wild type MT1-MMP or the recombinant 50 kDa enzyme form | Homo sapiens | ? | - |
? | |
pro-MMP-2 | MT1-MMP accomplishes full pro-MMP-2 activation, cleavage within the prodomain at the Asn37-Leu38 peptide bond | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane type 1 matrix metalloproteinase | - |
Homo sapiens |
MT1-MMP | - |
Homo sapiens |