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Literature summary for 3.4.24.78 extracted from
- Endopeptidase GPR (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 983-984.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is synthesized only during sporulation within the developing spore, and as a zymogen, ca. 2 h after its synthesis the zymogen autoprocesses to the active enzyme by intramolecular removal of 15 N-terminal residues | Priestia megaterium |
| synthesis | the enzyme is synthesized only during sporulation within the developing spore, and as a zymogen, ca. 2 h after its synthesis the zymogen autoprocesses to the active enzyme by intramolecular removal of 16 N-terminal residues | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Priestia megaterium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| at 3 A resolution | Priestia megaterium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gpr-negative mutants do not rapidly proceed to vegetative growth following spore germination, as transcription is inhibited by the undegraded small acid-soluble protein | Priestia megaterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by EDTA, 1,10-phenanthroline, DFP, N-ethylmaleimide, iodoacetamide or by inhibitors of aspartic proteases | Bacillus subtilis | |
| additional information | not inhibited by EDTA, 1,10-phenanthroline, DFP, N-ethylmaleimide, iodoacetamide or by inhibitors of aspartic proteases | Priestia megaterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
purified small acid-soluble protein | - |
Priestia megaterium | |
| 20 | - |
small tetra-to heptapeptides containing a cleavage site | - |
Priestia megaterium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000 | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| small acid-soluble protein + H2O | Bacillus subtilis | - |
? | - |
? |  |
| small acid-soluble protein + H2O | Priestia megaterium | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
- |
- |
| Bacillus cereus | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Halalkalibacterium halodurans | - |
- |
- |
| Oceanobacillus iheyensis | - |
- |
- |
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Priestia megaterium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | - |
Bacillus subtilis | - |
| spore | - |
Priestia megaterium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GPR zymogen + H2O | - |
Bacillus subtilis | ? | - |
? | |
| GPR zymogen + H2O | - |
Priestia megaterium | ? | - |
? | |
| purified small acid-soluble protein + H2O | - |
Priestia megaterium | ? | - |
? | |
| small acid-soluble protein + H2O | - |
Bacillus subtilis | ? | - |
? | |
| small acid-soluble protein + H2O | - |
Priestia megaterium | ? | - |
? | |
| small tetra-to heptapeptides containing a cleavage site + H2O | - |
Priestia megaterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 40000 | Priestia megaterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| germination proteinase | - |
Bacillus subtilis |
| germination proteinase | - |
Priestia megaterium |
| germination proteinase | - |
Bacillus cereus |
| germination proteinase | - |
Halalkalibacterium halodurans |
| germination proteinase | - |
Bacillus anthracis |
| germination proteinase | - |
Oceanobacillus iheyensis |
| GPR | - |
Bacillus subtilis |
| GPR | - |
Priestia megaterium |
| GPR | - |
Bacillus cereus |
| GPR | - |
Halalkalibacterium halodurans |
| GPR | - |
Bacillus anthracis |
| GPR | - |
Oceanobacillus iheyensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Priestia megaterium |
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