Literature summary for 3.4.24.75 extracted from

Boksha, I.S.; Lavrova, N.V.; Grishin, A.V.; Demidenko, A.V.; Lyashchuk, A.M.; Galushkina, Z.M.; Ovchinnikov, R.S.; Umyarov, A.M.; Avetisian, L.R.; Chernukha, M.I.u.; Shaginian, I.A.; Lunin, V.G.; Karyagina, A.S.
Staphylococcus simulans recombinant lysostaphin production, purification, and determination of antistaphylococcal activity (2016), Biochemistry, 81, 502-510 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Staphylococcus simulans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptidoglycan + H2O	Staphylococcus simulans	peptidoglycans from cell walls of Staphylococcus aureus and Staphylococcus haemolyticus	fragments of peptidogylcan	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus simulans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Unosphere S column chromatography	Staphylococcus simulans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptidoglycan + H2O	peptidoglycans from cell walls of Staphylococcus aureus and Staphylococcus haemolyticus	Staphylococcus simulans	fragments of peptidogylcan	-	?

Subunits

Subunits	Comment	Organism
?	x * 27500, SDS-PAGE	Staphylococcus simulans
?	x * 27517, calculated from amino acid sequence	Staphylococcus simulans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Staphylococcus simulans

General Information

General Information	Comment	Organism
physiological function	the enzyme lyses staphylococcus cell walls by cleaving pentaglycine cross_bridges in their peptidoglycan	Staphylococcus simulans

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Release 2023.1 (January 2023)