Literature summary for 3.4.24.74 extracted from

Kharlampieva, D.; Manuvera, V.; Podgorny, O.; Grafskaia, E.; Kovalchuk, S.; Pobeguts, O.; Altukhov, I.; Govorun, V.; Lazarev, V.
Recombinant fragilysin isoforms cause E-cadherin cleavage of intact cells and do not cleave isolated E-cadherin (2015), Microb. Pathog., 83-84, 47-56.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Gold cells	Bacteroides fragilis

Protein Variants

Protein Variants	Comment	Organism
E349A	no activity with E-cadherin	Bacteroides fragilis
H348Y/H352Y/H358Y	no activity with E-cadherin	Bacteroides fragilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains zinc	Bacteroides fragilis

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	O86049	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni Sepharose column chromatography and Superdex 200 gel filtration	Bacteroides fragilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
E-cadherin + H2O	recombinant fragilysin isoforms cause E-cadherin cleavage of intact cells and do not cleave isolated E-cadherin	Bacteroides fragilis	?	-	?
additional information	the recombinant enzyme does not cleave thioredoxin, gelatin, azocoll or azocasein both with and without Zn2+ ions	Bacteroides fragilis	?	-	?

Synonyms

Synonyms	Comment	Organism
BFT	-	Bacteroides fragilis

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Release 2023.1 (January 2023)