Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.72 extracted from

  • Nascimento, T.; Sales, A.; Porto, C.; Brandao, R.; de Campos-Takaki, G.; Teixeira, J.; Porto, T.; Porto, A.; Converti, A.
    Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aqueous two-phase systems (PEG/sulfate) (2016), J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 1025, 16-24 .
    View publication on PubMed
Search for more literature for 3.4.24.72

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 61.59% residual activity at 10 mM Mucor subtilissimus
Cu2+ 54.09% residual activity at 10 mM Mucor subtilissimus
Fe2+ 52.95% residual activity at 10 mM Mucor subtilissimus
K+ 93.86% residual activity at 2.5 mM Mucor subtilissimus
additional information not inhibited by EDTA, iodoacetic acid, and 2-mercaptoethanol Mucor subtilissimus
Na+ 91.59% residual activity at 2.5 mM Mucor subtilissimus
pepstatin A 91.3% residual activity at 5 mM Mucor subtilissimus
phenylmethylsulfonyl fluoride complete inhibition at 5 mM Mucor subtilissimus
Zn2+ 30% residual activity at 10 mM Mucor subtilissimus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 119.09% activity at 2.5 mM Mucor subtilissimus
Mg2+ 110.91% residual activity at 10 mM Mucor subtilissimus
Mn2+ 109.31% residual activity at 2.5 mM Mucor subtilissimus

Organism

Organism UniProt Comment Textmining
Mucor subtilissimus
-
-
-
Mucor subtilissimus UCP 1262
-
-
-

Purification (Commentary)

Purification (Comment) Organism
aqueous two-phase system with 30% (w/v) polyethylene glycol 6000 and 13.2% (w/v) sodium sulfate Mucor subtilissimus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Mucor subtilissimus ?
-
 ?
Fibrin + H2O
-
 Mucor subtilissimus ?
-
 ?
additional information no activity with Gly-Arg-4-nitroanilide and Nalpha-benzoyl-DL-Arg-4-nitroanilide Mucor subtilissimus ?
-
 ?
additional information no activity with Gly-Arg-4-nitroanilide and Nalpha-benzoyl-DL-Arg-4-nitroanilide Mucor subtilissimus UCP 1262 ?
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Mucor subtilissimus succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 97000, SDS-PAGE Mucor subtilissimus

Synonyms

Synonyms Comment Organism
fibrinolytic protease
-
 Mucor subtilissimus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mucor subtilissimus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 the enzyme shows more than 80% activity between 10 and 60°C. The enzyme is completely denatured at temperatures above 80°C Mucor subtilissimus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 at 37°C, the enzyme residual activity is 94 and 68% of the initial one after 120 and 150 min of incubation, respectively Mucor subtilissimus

pI Value

Organism Comment pI Value Maximum pI Value
Mucor subtilissimus isoelectric focusing
-
 5.4