Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.72 extracted from

  • Sun, Z.; Liu, P.; Cheng, G.; Zhang, B.; Dong, W.; Su, X.; Huang, Y.; Cui, Z.; Kong, Y.
    A fibrinolytic protease AfeE from Streptomyces sp. CC5, with potent thrombolytic activity in a mouse model (2016), Int. J. Biol. Macromol., 85, 346-354 .
    View publication on PubMed
Search for more literature for 3.4.24.72

Application

Application Comment Organism
medicine the enzyme is a candidate for antithrombotic drug development Streptomyces sp. CC5

Inhibitors

Inhibitors Comment Organism Structure
aprotin 84.1% residual activity at 1 mM Streptomyces sp. CC5
Co2+ 60.4% residual activity at 1 mM Streptomyces sp. CC5
Cu2+ 50.1% residual activity at 1 mM Streptomyces sp. CC5
EDTA 77.5% residual activity at 5 mM Streptomyces sp. CC5
EGTA 74.7% residual activity at 5 mM Streptomyces sp. CC5
Fe2+ 77.3% residual activity at 1 mM Streptomyces sp. CC5
leupeptin 98.6% residual activity at 1 mM Streptomyces sp. CC5
Mn2+ 94.2% residual activity at 1 mM Streptomyces sp. CC5
Na+ 97.7% residual activity at 1 mM Streptomyces sp. CC5
pepstatin A 95.2% residual activity at 1 mM Streptomyces sp. CC5
phenylmethylsulfonyl fluoride 18.5% residual activity at 1 mM Streptomyces sp. CC5
Soybean trypsin inhibitor 28.2% residual activity at 1 mM Streptomyces sp. CC5
tosyl-L-lysine chloromethyl ketone 21.3% residual activity at 0.1 mM Streptomyces sp. CC5
tosyl-L-phenylalanine chloromethyl ketone 37.2% residual activity at 0.1 mM Streptomyces sp. CC5
Zn2+ 67.6% residual activity at 1 mM Streptomyces sp. CC5

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 108.7% activity at 1 mM Streptomyces sp. CC5
Mg2+ 112.6% activity at 1 mM Streptomyces sp. CC5
additional information not influenced by K+ Streptomyces sp. CC5

Organism

Organism UniProt Comment Textmining
Streptomyces sp. CC5 A0A0A7W604
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HPD-100 macroporous resin column chromatography, 201 × 7 ion-exchange resin column chromatography, and Superdex 200 gel filtration Streptomyces sp. CC5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
albumin from chicken egg + H2O lowest activity Streptomyces sp. CC5 ?
-
 ?
Bovine serum albumin + H2O
-
 Streptomyces sp. CC5 ?
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
D-Phe-Pip-Arg-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Phe-Pip-Arg + 4-nitroaniline
-
 ?
D-Val-Leu-Lys-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Val-Leu-Lys + 4-nitroaniline
-
 ?
Fibrin + H2O best substrate. The enzyme exhibits about 2fold higher activity for fibrin than fibrinogen. Fibrin can be hydrolyzed completely in about 30 min Streptomyces sp. CC5 ?
-
 ?
fibrinogen + H2O fibrinogen can be hydrolyzed completely in 4-6 h Streptomyces sp. CC5 fibrin + ?
-
 ?
Hemoglobin + H2O
-
 Streptomyces sp. CC5 ?
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O second best substrate Streptomyces sp. CC5 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 29984, high resolution mass spectrometry Streptomyces sp. CC5

Synonyms

Synonyms Comment Organism
AfeE
-
 Streptomyces sp. CC5
fibrinolytic protease
-
 Streptomyces sp. CC5

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
-
 Streptomyces sp. CC5

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 60 the enzyme maintains up to 80% of its maximum activity at a temperature range of 37-60°C Streptomyces sp. CC5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 the enzyme activity remains stable for 60 min at 20-30°C and loses about 50% activity after 60 min at 50°C. After 60 min at 60°C, the enzyme completely loses its activity Streptomyces sp. CC5

pH Range

pH Minimum pH Maximum Comment Organism
6 12 the enzyme shows more than 60% activity at pH values ranging from 6.0 to 12.0 Streptomyces sp. CC5

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 the enzyme activity remains stable after 60 min at pH 6.0-12.0 Streptomyces sp. CC5