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Literature summary for 3.4.24.72 extracted from

  • Silva, G.; Bezerra, R.; Teixeira, J.; Silva, F.; Correia, J.; Porto, T.; Lima-Filho, J.; Porto, A.
    Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens (2016), Acta Amaz., 46, 301-310 .
No PubMed abstract available
Search for more literature for 3.4.24.72

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 88.8% residual activity at 1 mM Streptomyces sp. DPUA 1576
Co2+ 60.8% residual activity at 1 mM Streptomyces sp. DPUA 1576
Cu2+ 34.2% residual activity at 1 mM Streptomyces sp. DPUA 1576
EDTA 39.9% residual activity at 10 mM Streptomyces sp. DPUA 1576
Fe2+ 95.3% residual activity at 1 mM Streptomyces sp. DPUA 1576
Fe3+ 95.4% residual activity at 1 mM Streptomyces sp. DPUA 1576
Mg2+ 94.4% residual activity at 1 mM Streptomyces sp. DPUA 1576
additional information not inhibited by iodoacetic acid Streptomyces sp. DPUA 1576
pepstatin A 14.6% residual activity at 1 mM Streptomyces sp. DPUA 1576
phenylmethylsulfonyl fluoride 25.4% residual activity at 50 mM Streptomyces sp. DPUA 1576
Zn2+ 81.4% residual activity at 1 mM Streptomyces sp. DPUA 1576

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Mn2+ Streptomyces sp. DPUA 1576
Na+ the activity of protease is slightly enhanced (to 106%) by the presence of Na+ ions Streptomyces sp. DPUA 1576

Organism

Organism UniProt Comment Textmining
Streptomyces sp. DPUA 1576
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Streptomyces sp. DPUA 1576 ?
-
 ?
Fibrin + H2O
-
 Streptomyces sp. DPUA 1576 ?
-
 ?
fibrinogen + H2O
-
 Streptomyces sp. DPUA 1576 fibrin + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Streptomyces sp. DPUA 1576

Synonyms

Synonyms Comment Organism
fibrinolytic enzyme
-
 Streptomyces sp. DPUA 1576

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 highest fibrinolytic activity Streptomyces sp. DPUA 1576

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 highest protease activity between 35 and 55°C. The relative activities at 25°C and 75°C are 59% and 49%, respectively Streptomyces sp. DPUA 1576

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 75 the enzyme is stable during 120 min at 25 and 35°C and shows 98, 100, and 59% residual activity at 25, 35 and 45°C, respectively. The enzyme in the crude extract loses its activity after 60 min between 55 and 75°C Streptomyces sp. DPUA 1576

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 optimum pH for the fibrinolytic activity Streptomyces sp. DPUA 1576

pH Range

pH Minimum pH Maximum Comment Organism
7 8 the enzyme activity decreases rapidly at levels below pH 7.0 and above pH 8.0 Streptomyces sp. DPUA 1576

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 9.2 protease and fibrinolytic activities are stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively Streptomyces sp. DPUA 1576