Cloned (Comment) | Organism |
---|---|
gene alf, high level expression of His-tagged alfimeprase in Pichia pastoris strains of GS115 and KM71 by methanol induction, secretion of the recombinant enzyme to the cell culture medium | Agkistrodon contortrix contortrix |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Agkistrodon contortrix contortrix | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23800 | - |
x * 23800, recombinant His-tagged alfimeprase, SDS-PAGE | Agkistrodon contortrix contortrix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | Agkistrodon contortrix contortrix | fibrinolytic activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agkistrodon contortrix contortrix | P28891 | gene alf | - |
Purification (Comment) | Organism |
---|---|
secreted recombinant His-tagged alfimeprase from Pichia pastoris strains of GS115 and KM71 cell culture medium by immunoaffinity chromatography to 96% purity | Agkistrodon contortrix contortrix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Agkistrodon contortrix contortrix | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | fibrinolytic activity | Agkistrodon contortrix contortrix | ? | - |
? | |
additional information | the enzyme is a nonhemorrhagic zinc metalloprotease | Agkistrodon contortrix contortrix | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23800, recombinant His-tagged alfimeprase, SDS-PAGE | Agkistrodon contortrix contortrix |
Synonyms | Comment | Organism |
---|---|---|
ALF | - |
Agkistrodon contortrix contortrix |
Alfimeprase | - |
Agkistrodon contortrix contortrix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Agkistrodon contortrix contortrix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Agkistrodon contortrix contortrix |